Anti-PDCD4 (phospho S457) 抗体 [9G6] (ab105998)
![Western blot - Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)](https://www.abcam.co.jp/ps/products/105/ab105998/Images/ab105998-118372-anti-pdcd4-phospho-s457-antibody-9g6-western-blot.jpg "Western blot - Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)")
Key features and details
- Mouse monoclonal [9G6] to PDCD4 (phospho S457)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-PDCD4 (phospho S457) antibody [9G6]
PDCD4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [9G6] to PDCD4 (phospho S457) -
由来種
Mouse -
特異性
This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human PDCD4 (phospho S457).
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ポジティブ･コントロール
- NIH-3T3 cells stimulated with PDGF, Recombinant PDCD4 protein, Human small intestine tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Pdcd4 phospho S457 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. -
ポリ/モノ
モノクローナル -
クローン名
9G6 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105998の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
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| IHC-P |
1/1000 - 1/5000.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 62 kDa. |
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IHC-P
1/1000 - 1/5000. |
ターゲット情報
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機能
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA. -
組織特異性
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. -
配列類似性
Belongs to the PDCD4 family.
Contains 2 MI domains. -
ドメイン
Binds EIF4A1 via both MI domains. -
翻訳後修飾
Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. -
細胞内局在
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum. - Information by UniProt
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参照データベース
- Entrez Gene: 27250 Human
- Entrez Gene: 18569 Mouse
- Entrez Gene: 64031 Rat
- Omim: 608610 Human
- SwissProt: Q53EL6 Human
- SwissProt: Q61823 Mouse
- SwissProt: Q9JID1 Rat
- Unigene: 711490 Human
see all -
別名
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
see all
画像
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Western blot - Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)All lanes : Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998) at 1/2000 dilution
Lane 1 : Unstimulated (non-phosphorylated) NIH 3T3 cells
Lane 2 : NIH-3T3 cells stimulated with 30 ng/ml PDGF (5 min treatment)
Secondary
All lanes : HRP conjugated Rabbit anti-Mouse IgG at 1/20000 dilution
Predicted band size: 62 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)](https://www.abcam.co.jp/ps/products/105/ab105998/Images/ab105998-118371-anti-pdcd4-phospho-s457-antibody-9g6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)ab105998 at 1/1000 dilution staining PDCD4 in Human small intestine by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
The image shows the localization of ab105998 as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab105998 は 3 報の論文で使用されています。
- Kenawy N et al. Conjunctival melanoma copy number alterations and correlation with mutation status, tumor features, and clinical outcome. Pigment Cell Melanoma Res 32:564-575 (2019). PubMed: 30672666
- Yuan H et al. Downregulation of PDCD4 by miR-21 suppresses tumor transformation and proliferation in a nude mouse renal cancer model. Oncol Lett 14:3371-3378 (2017). PubMed: 28927090
- Mao XH et al. MicroRNA-21 regulates the ERK/NF-?B signaling pathway to affect the proliferation, migration, and apoptosis of human melanoma A375 cells by targeting SPRY1, PDCD4, and PTEN. Mol Carcinog 56:886-894 (2017). PubMed: 27533779