Anti-PD1 抗体 [EPR26302-8] (ab300425)
![Flow Cytometry - Anti-PD1 antibody [EPR26302-8] (AB300425)](https://www.abcam.co.jp/ps/products/300/ab300425/Images/AB300425-3-anti-pd1-antibody-epr263028-flow-cytometry-el-4.jpg "Flow Cytometry - Anti-PD1 antibody [EPR26302-8] (AB300425)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26302-8] to PD1
- Suitable for: IP, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-PD1 antibody [EPR26302-8]
PD1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR26302-8] to PD1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, ICC/IF, Flow Cyt, WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse
非交差種: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: EL4 whole cell lysate. ICC/IF: EL4 cells. Flow Cyt: EL4 cells. IP: EL4 cell whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR26302-8 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300425の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
1/30.
|
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| ICC/IF |
1/100.
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| Flow Cyt |
1/500.
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| WB |
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 31 kDa).
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| 特記事項 |
|---|
|
IP
1/30. |
|
ICC/IF
1/100. |
|
Flow Cyt
1/500. |
|
WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 31 kDa). |
ターゲット情報
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機能
Possible cell death inducer, in association with other factors. -
関連疾患
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
発生段階
Induced at programmed cell death. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 18566 Mouse
- SwissProt: Q02242 Mouse
- Unigene: 5024 Mouse
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別名
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
see all
画像
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Flow Cytometry - Anti-PD1 antibody [EPR26302-8] (AB300425)
Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Right) cells labeling PD-1 with ab300425 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: CTLL-2 (left) (PMID: 1396582).
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![Western blot - Anti-PD1 antibody [EPR26302-8] (AB300425)](https://www.abcam.co.jp/ps/products/300/ab300425/Images/AB300425-486726-anti-pd1-antibody-epr263028-western-blot-el4-mouse-lymphoma-t-lymphocyte-whole-cell-lysate-40-ug.jpg)
Western blot - Anti-PD1 antibody [EPR26302-8] (AB300425)All lanes : Anti-PD1 antibody [EPR26302-8] (ab300425) at 1/1000 dilution
Lane 1 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate
Lane 2 : CTLL-2 (mouse T lymphocyte), whole cell lysate
Lane 3 : L929 (mouse connective tissue fibroblast), whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 34172932).
Negative controls: CTLL-2, L929 (PMID: 1396582)
Exposure time: 26 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR26302-8] (ab300425)](https://www.abcam.co.jp/ps/products/300/ab300425/Images/AB300425-4-anti-pd1-antibody-epr263028-immunocytochemistry-el4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PD1 antibody [EPR26302-8] (ab300425)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labeling PD-1 with ab300425 at 1/100 dilution (4.47 µg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in EL4 cell line is observed. Negative control: CTLL-2 (PMID: 34580467). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: PBS was used instead of primary antibody follwed by preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Immunoprecipitation - Anti-PD1 antibody [EPR26302-8] (AB300425)](https://www.abcam.co.jp/ps/products/300/ab300425/Images/AB300425-2-anti-pd1-antibody-epr263028-immunoprecipitation-el4.jpg)
Immunoprecipitation - Anti-PD1 antibody [EPR26302-8] (AB300425)PD-1 was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte), whole cell lysate 10 ug with ab300425 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300425 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: EL4 (mouse lymphoma T lymphocyte), whole cell lysate 10 ug
Lane 2: ab300425 IP in EL4 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300425 in EL4 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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![Anti-PD1 antibody [EPR26302-8] (AB300425)](https://www.abcam.co.jp/ps/products/300/ab300425/Images/AB300425-5-Benefits-of-Recombinant-antibodies.jpg)
Anti-PD1 antibody [EPR26302-8] (AB300425)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300425 は論文での使用が確認できていません。