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リコンビナントRabMAb

Anti-PCTP 抗体 [EPR15390] (ab184944)

Submit a review Submit a question References (1)
Western blot - Anti-PCTP antibody [EPR15390] (ab184944)
  • Flow Cytometry (Intracellular) - Anti-PCTP antibody [EPR15390] (ab184944)
  • Immunoprecipitation - Anti-PCTP antibody [EPR15390] (ab184944)
  • Anti-PCTP antibody [EPR15390] (ab184944)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15390] to PCTP
  • Suitable for: IP, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PCTP antibody [EPR15390]
    PCTP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15390] to PCTP

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WB, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal liver and K562 cell lysates, K562 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184944の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/30 - 1/50.
WB
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
IP
1/30 - 1/50.
WB
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Catalyzes the transfer of phosphatidylcholine between membranes. Binds a single lipid molecule.

  • 組織特異性

    Highest expression in liver, placenta, testis, kidney and heart. Low levels in brain and lung. No expression detected in thymus.

  • 配列類似性

    Contains 1 START domain.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • PC TP antibody
      • PC-TP antibody
      • PCTP antibody
      • Phosphatidylcholine transfer protein antibody
      • PPCT_HUMAN antibody
      • StAR related lipid transfer (START) domain containing 2 antibody
      • StAR related lipid transfer protein 2 antibody
      • StAR-related lipid transfer protein 2 antibody
      • STARD 2 antibody
      • StARD2 antibody
      • START domain containing 2 antibody
      • START domain containing protein 2 antibody
      • START domain-containing protein 2 antibody
      see all

    画像

    • Western blot - Anti-PCTP antibody [EPR15390] (ab184944)
      Western blot - Anti-PCTP antibody [EPR15390] (ab184944)
      All lanes : Anti-PCTP antibody [EPR15390] (ab184944) at 1/1000 dilution

      Lane 1 : Human fetal liver tissue lysate
      Lane 2 : K562 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 25 kDa
      Observed band size: 25 kDa

    • Flow Cytometry (Intracellular) - Anti-PCTP antibody [EPR15390] (ab184944)
      Flow Cytometry (Intracellular) - Anti-PCTP antibody [EPR15390] (ab184944)

      Intracellular Flow Cytometry analysis of paraformaldehyde-fixed K562 cells labeling PCTP with ab184944 at a 1/200 dilution (Red), with a negative control rabbit IgG (Green). Secondary antibody goat anti-rabbit IgG (FITC) at a 1/150 dilution. 

       

       

    • Immunoprecipitation - Anti-PCTP antibody [EPR15390] (ab184944)
      Immunoprecipitation - Anti-PCTP antibody [EPR15390] (ab184944)

      Western blot analysis on immunoprecipitation from Human fetal liver lysate (Lane 1), labeling PCTP using ab184944 at 1/50 dilution Lane 2 shows PBS negative control. Secondary antibody, HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

    • Anti-PCTP antibody [EPR15390] (ab184944)
      Anti-PCTP antibody [EPR15390] (ab184944)

    参考文献 (1)

    ab184944 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184944 は 1 報の論文で使用されています。

    • James-Allan LB  et al. Changes in Placental Nutrient Transporter Protein Expression and Activity Across Gestation in Normal and Obese Women. Reprod Sci 27:1758-1769 (2020). PubMed: 32072607

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