Anti-PCTAIRE1 抗体 [EPR13210] (ab181208)
![Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)](https://www.abcam.co.jp/ps/products/181/ab181208/Images/ab181208-269779-ab181208KOWB.jpg "Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13210] to PCTAIRE1
- Suitable for: WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PCTAIRE1 antibody [EPR13210]
PCTAIRE1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13210] to PCTAIRE1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- BxPC-3, Human testis and Human fetal heart cell lysates; BxPC-3 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13210 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181208の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 62, 68 kDa (predicted molecular weight: 56 kDa).
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| Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 62, 68 kDa (predicted molecular weight: 56 kDa). |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
May play a role in signal transduction cascades in terminally differentiated cells. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 5127 Human
- Entrez Gene: 18555 Mouse
- Entrez Gene: 81741 Rat
- Omim: 311550 Human
- SwissProt: Q00536 Human
- SwissProt: Q04735 Mouse
- SwissProt: Q63686 Rat
- Unigene: 496068 Human
see all -
別名
- Cdk16 antibody
- CDK16_HUMAN antibody
- Cell division protein kinase 16 antibody
see all
画像
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Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PCTAIRE1 knockout HAP1 cell lysate (20 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181208 observed at 63 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab181208 was shown to recognize PCTAIRE1 when PCTAIRE1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PCTAIRE1 knockout samples were subjected to SDS-PAGE. ab181208 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)](https://www.abcam.co.jp/ps/products/181/ab181208/Images/ab181208-215116-ab1812083.jpg)
Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)Intracellular flow cytometric analysis of BxPC-3cells (2% paraformaldehyde-fixed) labeling PCTAIRE1with ab181208 at 1/20 dilution (red) or a rabbit IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.
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![Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)](https://www.abcam.co.jp/ps/products/181/ab181208/Images/ab181208-215115-ab1812082.jpg)
Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)All lanes : Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution
Lane 1 : Human testis cell lysate
Lane 2 : Human fetal heart cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 56 kDaThese two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.
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![Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)](https://www.abcam.co.jp/ps/products/181/ab181208/Images/ab181208-215114-ab1812081.jpg)
Western blot - Anti-PCTAIRE1 antibody [EPR13210] (ab181208)Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution + BxPC-3 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 56 kDaThese two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.
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![Anti-PCTAIRE1 antibody [EPR13210] (ab181208)](https://www.abcam.co.jp/ps/products/181/ab181208/Images/ab181208-5-benefits-of-recombinant-antibodies.png)
Anti-PCTAIRE1 antibody [EPR13210] (ab181208)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab181208 は論文での使用が確認できていません。