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AB238995

Anti-PCSK9 抗体 [EPR7627(2)] - BSA and Azide free

Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal PCSK9 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

別名を表示する

NARC1, PSEC0052, PCSK9, Proprotein convertase subtilisin/kexin type 9, Neural apoptosis-regulated convertase 1, Proprotein convertase 9, Subtilisin/kexin-like protease PC9, NARC-1, PC9

3 Images
Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)
  • IP

Supplier Data

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)

Western blot analysis of PCSK9 in HepG2 cell lysate immunoprecipitated using unpurified ab181142 at a dilution of 1/50.

Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181142).

All lanes:

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (<a href='/products/primary-antibodies/pcsk9-antibody-epr76272-ab181142'>ab181142</a>)

Predicted band size: 74 kDa

Observed band size: 62 kDa

false

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)
  • IP

Lab

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)

ab181142 (purified) at 1/50 immunoprecipitating PCSK9 in HepG2 whole cell lysate.

Lane 1 (input) : HepG2 whole cell lysate (10µg)

Lane 2 (+) : ab181142 + HepG2 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab181142 in HepG2 whole cell lysate.

For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181142).

All lanes:

Immunoprecipitation - Anti-PCSK9 antibody [EPR7627(2)] (<a href='/products/primary-antibodies/pcsk9-antibody-epr76272-ab181142'>ab181142</a>)

Predicted band size: 74 kDa

Observed band size: 62 kDa,78 kDa

false

Western blot - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)
  • WB

Supplier Data

Western blot - Anti-PCSK9 antibody [EPR7627(2)] - BSA and Azide free (AB238995)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab181142).

All lanes:

Western blot - Anti-PCSK9 antibody [EPR7627(2)] (<a href='/products/primary-antibodies/pcsk9-antibody-epr76272-ab181142'>ab181142</a>) at 1/10000 dilution

Lane 1:

A431 (Human epidermoid carcinoma cell line) cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 74 kDa

Observed band size: 62 kDa,78 kDa

false

関連する標識済み抗体及び組成の異なる製品 (2)

  • Unconjugated

    Anti-PCSK9 antibody [EPR7627(2)]

  • HRP

    HRP Anti-PCSK9 antibody [EPR7627(2)]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR7627(2)

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

ab238995 is the carrier-free version of ab181142.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protein responsible for the regulation of cholesterol levels by binding to low-density lipoprotein receptors (LDLR) on hepatocytes. This interaction marks the LDLRs for degradation reducing the liver's ability to clear LDL cholesterol from the blood. PCSK9 has a molecular weight of approximately 74 kDa. The protein is mainly expressed in the liver but is also found in the intestine and kidneys. PCSK9 is referred to as NARC-1 standing for neural apoptosis-regulated convertase 1 highlighting its role in the liver's cholesterol management system.
Biological function summary

PCSK9 influences cholesterol homeostasis by its important role in degrading LDL receptors. It functions independently rather than as part of larger protein complexes. PCSK9 gains particular interest in therapeutic contexts where its inhibition can lead to increased LDLR levels and enhanced clearance of LDL cholesterol. Biotinylated PCSK9 and mouse PCSK9 variants provide significant tools for experimental study. Kits such as the PCSK9 ELISA kit enable detailed measurement of PCSK9 levels in blood samples providing insights into cholesterol metabolism dynamics.

Pathways

PCSK9 operates within the lipid metabolism pathway and the cholesterol biosynthesis pathway. The protein's activity affects the fate of LDL cholesterol within these pathways. It interacts with proteins such as apolipoprotein B (ApoB) which plays a central role in the structural component of LDL particles. The modulation of these pathways by PCSK9 highlights the significance of its function in maintaining cardiovascular health and managing cholesterol levels.

PCSK9 is closely linked to hypercholesterolemia and coronary artery disease. Mutations in PCSK9 can lead to autosomal dominant hypercholesterolemia due to its effect on LDL receptor degradation. Other proteins such as ApoB and LDLR are involved in these conditions tightly interacting with PCSK9's regulatory function. A better understanding of PCSK9's role offers potential therapeutic targets for cardiovascular disease interventions especially through the development of PCSK9 antibodies and PCSK9 assays that adjust cholesterol levels.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members : low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments (PubMed : 18039658). Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation (PubMed : 17461796, PubMed : 18197702, PubMed : 18799458, PubMed : 22074827). Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway (PubMed : 18660751). Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
See full target information PCSK9
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Circulation 148:144-158 PubMed37125593

2023

Orally Bioavailable Macrocyclic Peptide That Inhibits Binding of PCSK9 to the Low Density Lipoprotein Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Douglas G Johns,Louis-Charles Campeau,Puja Banka,An Bautmans,Tjerk Bueters,Elisabetta Bianchi,Danila Branca,Paul G Bulger,Inne Crevecoeur,Fa-Xiang Ding,Robert M Garbaccio,Erik D Guetschow,Yan Guo,Sookhee N Ha,Jennifer M Johnston,Hubert Josien,Eunkyung A Kauh,Kenneth A Koeplinger,Jeffrey T Kuethe,Eseng Lai,Christine L Lanning,Anita Y H Lee,Li Li,Anilkumar G Nair,Edward A O'Neill,S Aubrey Stoch,David A Thaisrivongs,Thomas J Tucker,Petr Vachal,Kristien van Dyck,Frederic P Vanhoutte,Bram Volckaert,Dennis G Wolford,Andy Xu,Tian Zhao,Dan Zhou,Susan Zhou,Xiaohong Zhu,Hratch J Zokian,Abbas M Walji,Harold B Wood

Nature 590:344-350 PubMed33505024

2021

Integrated spatial genomics reveals global architecture of single nuclei.

Applications

Unspecified application

Species

Unspecified reactive species

Yodai Takei,Jina Yun,Shiwei Zheng,Noah Ollikainen,Nico Pierson,Jonathan White,Sheel Shah,Julian Thomassie,Shengbao Suo,Chee-Huat Linus Eng,Mitchell Guttman,Guo-Cheng Yuan,Long Cai
View all publications
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代替製品

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組合せ製品

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Secondary Antibodies

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  • 5
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Primary Antibodies

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Primary Antibodies

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Abcam product promise

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