Anti-PCSK9 抗体 [EPR17827-117] (ab185194)
![Western blot - Anti-PCSK9 antibody [EPR17827-117] (ab185194)](https://www.abcam.co.jp/ps/products/185/ab185194/Images/ab185194-1-185194WB1.jpg "Western blot - Anti-PCSK9 antibody [EPR17827-117] (ab185194)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17827-117] to PCSK9
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-PCSK9 antibody [EPR17827-117]
PCSK9 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17827-117] to PCSK9 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details
適用なし: IHC-P -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Mouse kidney, liver and placenta tissue lysates; Hepa1-6 whole cell lysate. ICC/IF: Hepa1-6 cells. Flow: Hepa1-6 cells. IP: Hepa1-6 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17827-117 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185194の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
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| WB |
1/1000. Detects a band of approximately 78, 60 kDa (predicted molecular weight: 74 kDa).
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| ICC/IF |
1/100.
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| IP |
1/30.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/500. |
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WB
1/1000. Detects a band of approximately 78, 60 kDa (predicted molecular weight: 74 kDa). |
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ICC/IF
1/100. |
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IP
1/30. |
ターゲット情報
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機能
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. -
組織特異性
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. -
関連疾患
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant. -
配列類似性
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain. -
翻訳後修飾
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 100102 Mouse
- SwissProt: Q80W65 Mouse
- Unigene: 133268 Mouse
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別名
- Convertase subtilisin/kexin type 9 preproprotein antibody
- FH3 antibody
- HCHOLA3 antibody
see all
画像
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Western blot - Anti-PCSK9 antibody [EPR17827-117] (ab185194)All lanes : Anti-PCSK9 antibody [EPR17827-117] (ab185194) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse placenta tissue lysate
Lane 4 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 74 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1-3:3 minutes; Lane 4:6 seconds.
Blocking/diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26092101).
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![Immunocytochemistry/ Immunofluorescence - Anti-PCSK9 antibody [EPR17827-117] (ab185194)](https://www.abcam.co.jp/ps/products/185/ab185194/Images/ab185194-2-185194ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PCSK9 antibody [EPR17827-117] (ab185194)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling PCSK9 with ab185194 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in Hepa1-6 cell line is observed.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-PCSK9 antibody [EPR17827-117] (ab185194)](https://www.abcam.co.jp/ps/products/185/ab185194/Images/ab185194-4-185194FC1.jpg)
Flow Cytometry (Intracellular) - Anti-PCSK9 antibody [EPR17827-117] (ab185194)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling PCSK9 with ab185194 at 1/500 dilution (red) compared with aRabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-PCSK9 antibody [EPR17827-117] (ab185194)](https://www.abcam.co.jp/ps/products/185/ab185194/Images/ab185194-3-185194IP1.jpg)
Immunoprecipitation - Anti-PCSK9 antibody [EPR17827-117] (ab185194)PCSK9 was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate with ab185194 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab185194 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: Hepa1-6 whole cell lysate 10 µg (input).
Lane 2: ab185194 IP in Hepa1-6 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG in Hepa1-6 whole cell lsyate (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.78-kDa band represents proPCSK9 (PMID: 25210046).
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![Anti-PCSK9 antibody [EPR17827-117] (ab185194)](https://www.abcam.co.jp/ps/products/185/ab185194/Images/ab185194-5-benefits-of-recombinant-antibodies.png)
Anti-PCSK9 antibody [EPR17827-117] (ab185194)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (9)
ab185194 は 9 報の論文で使用されています。
- Yang QC et al. Targeting PCSK9 reduces cancer cell stemness and enhances antitumor immunity in head and neck cancer. iScience 26:106916 (2023). PubMed: 37305703
- Martino E et al. MiR-15b-5p and PCSK9 inhibition reduces lipopolysaccharide-induced endothelial dysfunction by targeting SIRT4. Cell Mol Biol Lett 28:66 (2023). PubMed: 37587410
- Shu Z et al. The Lin28b/Wnt5a axis drives pancreas cancer through crosstalk between cancer associated fibroblasts and tumor epithelium. Nat Commun 14:6885 (2023). PubMed: 37898598
- Yang D et al. Endothelial cell-specific deletion of a microRNA accelerates atherosclerosis. Atherosclerosis 350:9-18 (2022). PubMed: 35462240
- Qian Z & Zhao J Silencing YY1 Alleviates Ox-LDL-Induced Inflammation and Lipid Accumulation in Macrophages through Regulation of PCSK9/ LDLR Signaling. J Microbiol Biotechnol 32:1406-1415 (2022). PubMed: 36330745
- Zhu W et al. Metformin Ameliorates Hepatic Steatosis induced by olanzapine through inhibiting LXRα/PCSK9 pathway. Sci Rep 12:5639 (2022). PubMed: 35379885
- Liang J et al. Di'ao Xinxuekang Capsule Improves the Anti-Atherosclerotic Effect of Atorvastatin by Downregulating the SREBP2/PCSK9 Signalling Pathway. Front Pharmacol 13:857092 (2022). PubMed: 35571088
- Guo W et al. Self-Assembly of a Multifunction DNA Tetrahedron for Effective Delivery of Aptamer PL1 and Pcsk9 siRNA Potentiate Immune Checkpoint Therapy for Colorectal Cancer. ACS Appl Mater Interfaces 14:31634-31644 (2022). PubMed: 35817627
- Song F et al. Ubiquitinated ligation protein NEDD4L participates in MiR-30a-5p attenuated atherosclerosis by regulating macrophage polarization and lipid metabolism. Mol Ther Nucleic Acids 26:1303-1317 (2021). PubMed: 34853729