Anti-PCSK2 抗体 (ab3533)
Key features and details
- Rabbit polyclonal to PCSK2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PCSK2 antibody
PCSK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PCSK2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig -
免疫原
Synthetic peptide corresponding to Mouse PCSK2 aa 600-700.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3533の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/20 - 1/200.
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WB |
Use a concentration of 2 µg/ml.
This antibody detects an ~65 kDa protein representing Proprotein 2 from alpha TC 6 cell extract. |
特記事項 |
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ICC/IF
1/20 - 1/200. |
WB
Use a concentration of 2 µg/ml. This antibody detects an ~65 kDa protein representing Proprotein 2 from alpha TC 6 cell extract. |
ターゲット情報
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機能
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. -
配列類似性
Belongs to the peptidase S8 family. Furin subfamily. -
細胞内局在
Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules. - Information by UniProt
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参照データベース
- Entrez Gene: 281968 Cow
- Entrez Gene: 5126 Human
- Entrez Gene: 18549 Mouse
- Entrez Gene: 445533 Pig
- Entrez Gene: 25121 Rat
- Omim: 162151 Human
- SwissProt: Q9GLR0 Cow
- SwissProt: P16519 Human
see all -
別名
- KEX2 like endoprotease 2 antibody
- KEX2-like endoprotease 2 antibody
- NEC 2 antibody
see all
画像
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Anti-PCSK2 antibody (ab3533) at 1/500 dilution + Mouse cerebellum cell lysate at 25 µg
Observed band size: 65 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescent analysis of PCSK2 (green) showing staining in the cytoplasm of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab3533 は 6 報の論文で使用されています。
- Ducuing H et al. SlitC-PlexinA1 mediates iterative inhibition for orderly passage of spinal commissural axons through the floor plate. Elife 9:N/A (2020). PubMed: 33345773
- Kreutzberger AJB et al. Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2. Elife 9:N/A (2020). PubMed: 33164744
- Blanco AM et al. Goldfish adipocytes are pancreatic beta cell-like, glucose-responsive insulin-producing cells. J Cell Physiol 235:6875-6886 (2020). PubMed: 31989646
- Wu S et al. Apatinib inhibits tumor growth and angiogenesis in PNET models. Endocr Connect N/A:N/A (2018). PubMed: 30557852
- Du W et al. HID-1 is required for homotypic fusion of immature secretory granules during maturation. Elife 5:N/A (2016). WB, IF ; Mouse . PubMed: 27751232
- Gagnon J et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). PubMed: 18706454