Anti-PCNA 抗体 [PC10] (ab29)
Key features and details
- Mouse monoclonal [PC10] to PCNA
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Anti-PCNA antibody [PC10]
PCNA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [PC10] to PCNA -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Pigeon, Pig, Drosophila melanogaster, Monkey, Zebrafish, Thornback ray, Dogfish, Catshark -
免疫原
Fusion protein corresponding to PCNA. Protein A-PCNA fusion protein obtained from pC2T construct. This construct lacked 93 nucleotides at the 3' end of PCNA.
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ポジティブ・コントロール
- WB: HeLa, HEK293, A431 whole cell lysates, PC12, SV40LT-SMC, NIH 3T3, rat liver, rat heart. IHC-P: Human breast (Paget's disease of the nipple), mouse liver, mouse gut, rat spinal cord DRG, developing chick brain, zebrafish intestine, rat spleen, rat large intestine. IHC-Fr: mouse embryonic brain, HeLa. Flow Cyt: HeLa.
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特記事項
This antibody clone [PC10] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
PC10 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab29の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (28) |
1/10000 - 1/30000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (20) |
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
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ICC/IF | (14) |
Use a concentration of 1 - 5 µg/ml.
Methanol fixation recommended |
Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
1/10000 - 1/30000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa). |
ICC/IF
Use a concentration of 1 - 5 µg/ml. Methanol fixation recommended |
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
配列類似性
Belongs to the PCNA family. -
翻訳後修飾
Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
細胞内局在
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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参照データベース
- Entrez Gene: 373984 Chicken
- Entrez Gene: 515499 Cow
- Entrez Gene: 37290 Drosophila melanogaster
- Entrez Gene: 5111 Human
- Entrez Gene: 18538 Mouse
- Entrez Gene: 692192 Pig
- Entrez Gene: 25737 Rat
- Entrez Gene: 30678 Zebrafish
see all -
別名
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)This image is courtesy of an anonymous AbreviewImmunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde-fixed mouse brain tissue. Stained with ab29 at 1/10000 dilution. Secondary antibody used was ab150109 at 1/250 dilution. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% Normal Donkey Serum for 19 hours at 4°C. Antigen retrieval method was heat mediated, Tris/EDTA.
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All lanes : Anti-PCNA antibody [PC10] (ab29) at 1 µg/ml
Lane 1 : HeLa 20 ug
Lane 2 : PC12 20 ug
Lane 3 : SV40LT-SMC 20 ug
Lane 4 : NIH 3T3 20 ug
Lane 5 : Rat liver 20 ug
Lane 6 : Rat heart 20 ug
Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 29 kDaMerged signal (red and green). Green - ab29 observed at 32 kDa. Red - loading control, ab52866, observed at 50 kDa.
All samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab29 and ab52866(Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 ug/mL and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
Mouse monoclonal [PC10] to PCNA - Proliferation Marker (ab29) used in immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections; 1/6000 for 2h at RT) on human tissue sections (Paget's disease of the nipple). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Incubation time: Secondary Antibody: Biotin conjugated goat anti mouse Igs (1/200).
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All lanes : Anti-PCNA antibody [PC10] (ab29) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 4 minutes -
ab29 stained in HeLa cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab29 at 5 µg/ml and ab6046 (Rabbit polyclonal to beta tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150117 (pseudo-colored red) and ab150080 (colored green) used at 1 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
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Overlay histogram showing HeLa cells stained with ab29 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween 20 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29, 0.1μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)ab29 at 1/6000 staining mouse embryo (day 17) liver and gut tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in Tris buffer was performed. The tissue was blocked before incubation with the antibody for 2 hours. A biotinylated goat polyclonal antibody was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for PCNA antibody [PC10] - Proliferation Marker (ab29) on Rat Tissue sections (adult spinal cord DRG). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody used at 1/6000 for 2 minutes at RT. Secondary Antibody: Biotin labelled goat anti mouse Igs (1/200).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)This image is courtesy of Carl Hobbs, King's College London, United KingdomMouse monoclonal [PC10] to PCNA - Proliferation Marker (ab29) used in immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections; 1/6000 for 2h at RT) on E6 developing chick brain). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Incubation time: Secondary Antibody: Biotin conjugated goat anti mouse Igs (1/200). NB: This image shows developing brain/overlying skin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
Mouse monoclonal [PC10] to PCNA - Proliferation Marker (ab29) used in immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections;1/6000 for 2h at RT) on intestine of adult zebrafish). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Incubation time: Secondary Antibody: Biotin conjugated goat anti mouse Igs (1/200). NB: The crypt nuclei on this image of zebrafish intestine, are positive for the PCNA/PC10 clone conforming to accepted localisation data for PCNA in other species.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
IHC image of PCNA staining in rat large intestine formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab29, 0.025µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)
IHC image of PCNA staining in rat spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab29, 1/30,000 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)Gong, C. et al PLoS One. 2010 Dec 20;5(12):e15630. doi: 10.1371/journal.pone.0015630 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Aldehyde dehydrogenase (ALDH1) expression correlates with clinical outcome of breast cancer patients
(A) Immunohistochemical staining shows tumors with poor clinical response (progressive or stable disease, PD/SD) to neo-adjuvant chemotherapy express high ALDH1 (>20% positive cancer cells) in pre-chemotherapy samples, and tumors with partal response (PR) express low ALDH1 (≤20% positive cancer cells). High proliferating cell nuclear antigen (PCNA)(>25% positive cancer cells) and poor apoptosis are observed in tumors with PD/SD after neo-adjuvant chemotherapy. Representative images of ALDH1 (×200), PCNA (×200) and Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP labeling (TUNEL,×400).
PCNA is detected using ab29 at 1/100 dilution.
(From Figure 1A of Gong et al)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [PC10] (ab29)Crabbe et al PLoS One. 2015 May 11;10(5):e0126846. doi: 10.1371/journal.pone.0126846. eCollection 2015 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Annexin V and PCNA staining of decellularized lung scaffolds recellularized with (A) MSCs in static versus bioreactor conditions for 14 (panels A, B for annexin V, and a, b for PCNA) and 28 days (panels C, D for annexin V, and c, d for PCNA) (single cells), (B) MSC cell clusters in static conditions at 14 days (panel A for annexin V, and a for PCNA), (C) C10 cells in static (panel A for annexin V, a for PCNA) versus bioreactor (panel B for annexin V, b for PCNA) conditions for 11 days.
An inset in Fig 4Ab with higher magnification is shown to demonstrate that a majority of the cells stained positive for PCNA. Cell nuclei are labeled in blue; marker of interest is labeled in green. Magnifications are 400x. Overlap of cell nucleus and marker of interest can generate green or white color. For each condition, images are representative of the entire lung.
MSCs = Bone marrow-derived mouse mesenchymal stromal (stem) cells.
PCNA is detected using ab29 at 1/1000 dilution.
(From Figure 4 of Crabbe et al)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (736)
ab29 は 736 報の論文で使用されています。
- Shen L et al. Leptin secreted by adipocytes promotes EMT transition and endometrial cancer progression via the JAK2/STAT3 signalling pathway. Adipocyte 13:2293273 (2024). PubMed: 38090745
- Dali O et al. Transgenerational epigenetic effects imposed by neonicotinoid thiacloprid exposure. Life Sci Alliance 7:N/A (2024). PubMed: 37973188
- Man KF et al. SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma. Nat Commun 14:7863 (2023). PubMed: 38030644
- Luo SW et al. FOXP4 promotes proliferation of human spermatogonial stem cells. Asian J Androl 25:322-330 (2023). PubMed: 36018067
- Shi L et al. Engineered FGF19ΔKLB protects against intrahepatic cholestatic liver injury in ANIT-induced and Mdr2-/- mice model. BMC Biotechnol 23:43 (2023). PubMed: 37789318