AB19166

Anti-PCNA 抗体

Anti-PCNA antibody

Lab Essentials
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(20 Publications)

Rabbit Polyclonal PCNA antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 20 publications.

別名を表示する

Proliferating cell nuclear antigen, PCNA, Cyclin

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (AB19166)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (AB19166)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling PCNA with ab19166 at 1/10000 (0.1µg/ml). Detection : DAB.

Unknown

Western blot - Anti-PCNA antibody (AB19166)

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Western blot - Anti-PCNA antibody (ab19166) at 0.1 µg/mL

Lane 1:

HEK293T whole cell lysate at 50 µg

Lane 2:

NIH-3T3 whole cell lysate at 50 µg

Predicted band size: 29 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.

Biological function summary

This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

Pathways

PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed : 35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed : 24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair : Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed : 24695737).

See full target information PCNA

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文献 (20)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36834717

2023

Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Hwan Kim,Eui Hwa Jang,Ji-Yeon Ryu,Jiyong Lee,Jae Ho Kim,Wonhyoung Ryu,Young-Nam Youn

Frontiers in genetics 13:933416 PubMed36299576

2022

The interconnected relationships between middle ear bulla size, cavitation defects, and chronic otitis media revealed in a syndromic mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Juan M Fons,Natalie J Milmoe,Michael R G Dack,Leena Joshi,Hannah Thompson,Abigail S Tucker

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 70:335-356 PubMed35400231

2022

Differential Expression of Secretogranins II and III in Canine Adrenal Chromaffin Cells and Pheochromocytomas.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Gomi,Takahiro Nagumo,Kazushi Asano,Makoto Konosu,Tadashi Yasui,Seiji Torii,Masahiro Hosaka

Microscopy research and technique 85:1238-1247 PubMed34817902

2021

Microscopic focus on ependymal cells of the spinal cord of the one-humped camel (Camelus dromedarius): Histological, immunohistochemical, and transmission microscopic study.

Applications

Unspecified application

Species

Unspecified reactive species

Karam Roshdy,Kareem Morsy,Mohamed M A Abumandour

Microscopy research and technique 84:3116-3127 PubMed34289208

2021

Ultrastructural and histological descriptions of the oropharyngeal cavity of the rock pigeon Columba livia dakhlae with special refer to its adaptive dietary adaptations.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed M A Abumandour,Neveen E R El-Bakary,Eman R Elbealy,Attalla El-Kott,Kareem Morsy,Seham S Haddad,Naglaa Madkour,Ramadan M Kandyel

American journal of translational research 12:6464-6477 PubMed33194044

2020

Erythropoietin enhances meniscal regeneration and prevents osteoarthritis formation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Ning Fu,Hui-Wu Li,Na Du,Xu Liang,Shi-Hao He,Kuang-Jin Guo,Tian-Fang Li

Experimental and therapeutic medicine 20:167 PubMed33093905

2020

Glutamate receptor ionotropic, kainate 1 serves as a novel tumor suppressor of colorectal carcinoma and predicts clinical prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong Ren,Jingzheng Liu,Liqing Yao,Jian Li,Zhipeng Qi,Bing Li

Bioscience reports 40: PubMed32969473

2020

Angiotensin II enhances the proliferation of Natural Killer/T-cell lymphoma cells via activating PI3K/Akt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gui-Hua Zhang,Fa-An Miao,Jin-Ge Xu,Yan Zhang

Thoracic cancer 11:1180-1190 PubMed32147945

2020

Long non-coding RNA SNHG6 promotes the growth and invasion of non-small cell lung cancer by downregulating miR-101-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Li,Yongxin Jiang,Xudong Xiang,Quan Gong,Chunyan Zhou,Lijuan Zhang,Qianli Ma,Li Zhuang

Journal of cellular and molecular medicine 24:441-450 PubMed31721438

2019

LncRNA DLEU2 accelerates the tumorigenesis and invasion of non-small cell lung cancer by sponging miR-30a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Weiming Wu,Yonghong Zhao,Erji Gao,Yang Li,Xiang Guo,Tiancheng Zhao,Weiwei He,Huibiao Zhang

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