Anti-PCNA 抗体 (ab152112)
Key features and details
- Rabbit polyclonal to PCNA
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-PCNA antibody
PCNA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PCNA -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Pig
-
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-253 of PCNA (Uniprot: P12004).
-
ポジティブ･コントロール
- Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF7, NT2D1, PC3, U87 MG, NIH 3T3, JC, BCL1, PC12, A549 and SiHa whole cell lysates; HeLa cells; Human KYSE170 xenograft and Human breast carcinoma tissues.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab152112の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
|
|
| IHC-P | (2) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.
|
| ICC/IF |
1/100 - 1/1000.
|
|
| IP |
1/100 - 1/500.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8. |
|
ICC/IF
1/100 - 1/1000. |
|
IP
1/100 - 1/500. |
ターゲット情報
-
機能
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
配列類似性
Belongs to the PCNA family. -
翻訳後修飾
Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
細胞内局在
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
-
参照データベース
- Entrez Gene: 5111 Human
- Entrez Gene: 18538 Mouse
- Entrez Gene: 692192 Pig
- Entrez Gene: 25737 Rat
- Omim: 176740 Human
- SwissProt: P12004 Human
- SwissProt: P17918 Mouse
- SwissProt: P04961 Rat
see all -
別名
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
画像
-
Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : HCT116 whole cell lysate at 15 µg
Lane 2 : HCT116 whole cell lysate at 10 µg
Lane 3 : HCT116 whole cell lysate at 5 µg
Lane 4 : HCT116 whole cell lysate at 1 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Immunoprecipitation - Anti-PCNA antibody (ab152112)PCBP2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts with 5 µg ab152112. Western blot was performed from the immunoprecipitate using ab152112.
Lane 1: PCPB2 protein only.
Lane 2: IgG instead of ab152112 in HeLa whole cell extract.
Lane 3: ab152112 IP in HeLa whole cell extract.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Paraffin-embedded mouseliver tissue stained for PCNA using ab152112 at 1/500 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCNA with ab152112 at 1/500 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human KYSE170 xenograft tissue labeling PCNA with ab152112 at 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling PCNA with ab152112 at 1/500 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Rat hind brain tissue labeling PCNA with ab152112 at 1/500 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling PCNA with ab152112 at 1/500 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab152112)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PCNA with ab152112 at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab152112)Immunofluorescent analysis of methanol-fixed HeLa cells labeling PCNA with ab152112 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab152112)Immunofluorescent analysis of HeLa (Human epithelial cells from cervix adenocarcinoma cell line) labeling PCNA with ab152112 at 1/500 dilution.
Counterstain is Hoechst 33342 (blue).
-
Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : HeLa whole cell lyate
Lane 6 : HepG2 whole cell lysate
Lane 7 : H1299 whole cell lysate
Lane 8 : HCT116 whole cell lysate
Lane 9 : MCF7 whole cell lysate
Lane 10 : NT2D1 whole cell lysate
Lane 11 : PC3 whole cell lysate
Lane 12 : U87 MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/1000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)Anti-PCNA antibody (ab152112) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)All lanes : Anti-PCNA antibody (ab152112) at 1/3000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : NIH 3T3 whole cell lysate
Lane 3 : PC12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-PCNA antibody (ab152112)Anti-PCNA antibody (ab152112) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (30)
ab152112 は 30 報の論文で使用されています。
- Heise J et al. Protective Effects of Early Caffeine Administration in Hyperoxia-Induced Neurotoxicity in the Juvenile Rat. Antioxidants (Basel) 12:N/A (2023). PubMed: 36829854
- Shahine Y et al. Diosmin nanocrystal gel alleviates imiquimod-induced psoriasis in rats via modulating TLR7,8/NF-κB/micro RNA-31, AKT/mTOR/P70S6K milieu, and Tregs/Th17 balance. Inflammopharmacology 31:1341-1359 (2023). PubMed: 37010718
- Zhao Y et al. MicroRNA-495 suppresses pre-eclampsia via activation of p53/PUMA axis. Cell Death Discov 8:132 (2022). PubMed: 35338123
- Jin M et al. Circ_0011292 knockdown mitigates progression and drug resistance in PTX-resistant non-small-cell lung cancer cells by regulating miR-433-3p/CHEK1 axis. Thorac Cancer 13:1276-1288 (2022). PubMed: 35348291
- Xu B et al. Circ_0017274 acts on miR-637/CDX2 axis to facilitate cisplatin resistance in gastric cancer. Clin Exp Pharmacol Physiol 49:1105-1115 (2022). PubMed: 35748299