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リコンビナントRabMAb

Anti-PCK2 抗体 [EPR14223(B)] (ab181170)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
  • Western blot - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
  • Western blot - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
  • Anti-PCK2 antibody [EPR14223(B)] (ab181170)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14223(B)] to PCK2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PCK2 antibody [EPR14223(B)]
    PCK2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14223(B)] to PCK2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, MCF7 or A431 lysate, Human liver or Mouse kidney tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181170の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min.

ターゲット情報

  • 機能

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

  • パスウェイ

    Carbohydrate biosynthesis; gluconeogenesis.

  • 関連疾患

    Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD) [MIM:261650]. A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.

  • 配列類似性

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Mitochondrion.
  • Information by UniProt

  • 参照データベース

    • 別名

      • EC 4.1.1.32 antibody
      • GTP mitochondrial precursor antibody
      • HGNC:8725 antibody
      • mitochondrial antibody
      • Mitochondrial phosphoenolpyruvate carboxykinase 2 antibody
      • OTTHUMP00000164700 antibody
      • PCK2 antibody
      • PCKGM_HUMAN antibody
      • PE antibody
      • PEP carboxykinase antibody
      • PEPCK antibody
      • PEPCK deficiency mitochondrial antibody
      • PEPCK M antibody
      • PEPCK-M antibody
      • PEPCK2 antibody
      • Phosphoenolpyruvate carboxykinase [GTP] antibody
      • Phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody
      • Phosphoenolpyruvate carboxykinase 2 mitochondrial antibody
      • Phosphoenolpyruvate carboxylase antibody
      • Phosphopyruvate carboxylase antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14223(B)] (ab181170)

      Immunohistochemical analysis of paraffin embedded Mouse kidney tissue labeling PCK2 with ab181170 at a 1/250 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 antibody [EPR14223(B)] (ab181170)

      Immunohistochemical analysis of paraffin embedded Human liver tissue labeling PCK2 with ab181170 at a 1/250 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
      Western blot - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
      All lanes : Anti-PCK2 antibody [EPR14223(B)] (ab181170) at 1/1000 dilution

      Lane 1 : MCF7 lysate
      Lane 2 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 71 kDa

    • Western blot - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
      Western blot - Anti-PCK2 antibody [EPR14223(B)] (ab181170)
      Anti-PCK2 antibody [EPR14223(B)] (ab181170) at 1/10000 dilution + HepG2 lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 71 kDa

    • Anti-PCK2 antibody [EPR14223(B)] (ab181170)
      Anti-PCK2 antibody [EPR14223(B)] (ab181170)

    参考文献 (1)

    ab181170 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181170 は 1 報の論文で使用されています。

    • Niu D  et al. Protective Effects of Iridoid Glycoside from Corni Fructus on Type 2 Diabetes with Nonalcoholic Fatty Liver in Mice. Biomed Res Int 2021:3642463 (2021). PubMed: 33542919

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