Anti-PCIF1 抗体 [EPR24319-207] (ab300487)

Blocking / Diluting buffer: 5% NFDM/TBST.

Blocking / Diluting buffer: 5% NFDM/TBST.

Blocking / Diluting buffer: 5% NFDM/TBST.

Exposure times: Lane 1, 3: 59 seconds; Lane 2, 4: 3 minutes.

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling PCIF1 with ab300487 at 1/200 dilution (2.35 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on human testis. The section was incubated with ab300487 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling PCIF1 with ab300487 at 1/200 dilution (2.35 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on mouse liver. The section was incubated with ab300487 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cells) labelling PCIF1 with ab300487 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) (red) at 1/200 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cells) labelling PCIF1 with ab300487 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed 1/1000 dilution (green). Confocal image showing nuclear staining in MCF7 cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) (red) at 1/200 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblasts) labelling PCIF1 with ab300487 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed 1/1000 dilution (green). Confocal image showing nuclear staining in NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) (red) at 1/200 dilution.

PCIF1 was immunoprecipitated from 10 μg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab300487 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab300487 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1: HeLa (whole cell lysate 10 μg (Input).

Lane 2: ab300487 IP HeLa whole cell lysate. 

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300487 in HeLa whole cell lysate.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 84 seconds.

Observed MW(KDa): 85

PCIF1 was immunoprecipitated from 10 μg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate with ab300487 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab300487 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).

Lane 2: ab300487 IP NIH/3T3 whole cell lysate. 

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300487 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

Observed MW(KDa): 85

Flow Cytometry (Intracellular) analysis of MCF7 (human breast adenocarcinoma epithelial cells) labeling PCIF1 (red) with ab300487 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, (ab150081)) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730) Isotype control. Blue - (unlabeled control) - Cells without incubation with primary and secondary antibodies.

Flow Cytometry (Intracellular) analysis of NIH/3T3 (mouse embryonic fibroblasts) labeling PCIF1 (red) with ab300487 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, (ab150081)) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730) Isotype control. Blue - (unlabeled control) - Cells without incubation with primary and secondary antibodies.