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リコンビナントRabMAb

Anti-PCGF6 抗体 [EPR26301-72] (BSA and Azide free) (ab300060)

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Western blot - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)
  • Western blot - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (ab300060)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)
  • Flow Cytometry (Intracellular) - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (ab300060)
  • Immunoprecipitation - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)
  • Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26301-72] to PCGF6 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra), IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free)
    PCGF6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26301-72] to PCGF6 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, Flow Cyt (Intra), IPmore details
    適用なし: ChIP or IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, 293T, Human testis and Human thymus lysates. ICC/IF: 293T cells. Flow Cyt. Intra.: HEK-293T cell. IP: HEK-293 cell.

  • 特記事項

    ab300060 is a carrier free version of ab300059. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300060の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 50, 48 kDa (predicted molecular weight: 39 kDa).
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 50, 48 kDa (predicted molecular weight: 39 kDa).
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for ChIP or IHC-P.

ターゲット情報

  • 機能

    Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase.

  • 組織特異性

    Ubiquitous.

  • 配列類似性

    Contains 1 RING-type zinc finger.

  • 翻訳後修飾

    Phosphorylated during mitosis. Phosphorylated on Ser-30 by CDK7 in vitro.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • MBLR antibody
      • Mel18 and Bmi1 like RING finger protein antibody
      • Mel18 and Bmi1-like RING finger antibody
      • Pcgf6 antibody
      • PCGF6_HUMAN antibody
      • Polycomb group RING finger protein 6 antibody
      • RING finger protein 134 antibody
      • RNF134 antibody
      see all

    画像

    • Western blot - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)
      Western blot - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)
      All lanes : Anti-PCGF6 antibody [EPR26301-72] (ab300059) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 39 kDa
      Observed band size: 50, 48 kDa why is the actual band size different from the predicted?


      Exposure time: 59 seconds


      This data was developed using ab300059, the same antibody clone in a different buffer formulation. 

      5% NFDM/TBST was used as blocking and diluting buffer and concentration. 

      The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 28049731).
      This blot was developed using a higher sensitivity ECL substrate.

       

    • Western blot - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (ab300060)
      Western blot - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (ab300060)
      All lanes : Anti-PCGF6 antibody [EPR26301-72] (ab300059) at 1/1000 dilution

      Lane 1 : Human testis tissue lysate
      Lane 2 : Human thymus tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 39 kDa
      Observed band size: 50, 48 kDa why is the actual band size different from the predicted?



      This data was developed using ab300059, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 28049731).

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

      Exposure time: 

      Lane 1: 3 minutes

      Lane 2: 26 seconds

    • Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)
      Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)

      This data was developed using ab300059, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized 293T (human embryonic kidney epithelial cell) cells lebelling PCGF6 with ab300059 at 1/500 (0.914 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 µg/mL dilution (Green). Confocal image showing nuclear staining in 293T cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 µg/mL dilution.

    • Flow Cytometry (Intracellular) - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (ab300060)
      Flow Cytometry (Intracellular) - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (ab300060)

      This data was developed using ab300059, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells labelling PCGF6 with ab300059 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

    • Immunoprecipitation - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)
      Immunoprecipitation - Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)

      This data was developed using ab300059, the same antibody clone in a different buffer formulation.

      PCGF6 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 µg with ab300059 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300059 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 µg

      Lane 2: ab300059 in HEK-293 whole cell lysate

      Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab300059 in HEK-293 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 7.75 seconds

       

    • Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)
      Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free) (AB300060)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300060 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300060 は論文での使用が確認できていません。

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