Anti-PCCA 抗体 - C-terminal (ab187686)
Key features and details
- Rabbit polyclonal to PCCA - C-terminal
- Suitable for: IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PCCA antibody - C-terminal
PCCA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PCCA - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Fr, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human PCCA aa 650 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P05165 -
ポジティブ･コントロール
- WB: HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates. IHC-P: Human lung cancer and ovarian carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab187686 was affinity purified using an epitope specific to PCCA immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187686の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
1/500 - 1/2000.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
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| 特記事項 |
|---|
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IHC-Fr
1/500 - 1/2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 80 kDa. |
ターゲット情報
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パスウェイ
Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3. -
関連疾患
Defects in PCCA are the cause of propionic acidemia type I (PA-1) [MIM:606054]. PA-1 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein. -
配列類似性
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 418774 Chicken
- Entrez Gene: 738775 Chimpanzee
- Entrez Gene: 614302 Cow
- Entrez Gene: 476975 Dog
- Entrez Gene: 101127469 Gorilla
- Entrez Gene: 100062251 Horse
- Entrez Gene: 5095 Human
- Entrez Gene: 110821 Mouse
see all -
別名
- mitochondrial antibody
- Pcca antibody
- pccA COMPLEMENTATION GROUP antibody
see all
画像
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Western blot - Anti-PCCA antibody - C-terminal (ab187686)All lanes : Anti-PCCA antibody - C-terminal (ab187686) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Frozen sections) - Anti-PCCA antibody - C-terminal (ab187686)Immunohistochemistry of human lung cancer tissue staining PCCA with an187686 at 1:1000. Detected using DAB.
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Immunohistochemistry (Frozen sections) - Anti-PCCA antibody - C-terminal (ab187686)Immunohistochemistry of human ovarian carcinoma tissue staining PCCA with an187686 at 1:1000. Detected using DAB.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab187686 は 4 報の論文で使用されています。
- Chandler RJ et al. Systemic gene therapy using an AAV44.9 vector rescues a neonatal lethal mouse model of propionic acidemia. Mol Ther Methods Clin Dev 30:181-190 (2023). PubMed: 37746248
- Hauser S et al. Application of a Fluorescence Anisotropy-Based Assay to Quantify Transglutaminase 2 Activity in Cell Lysates. Int J Mol Sci 23:N/A (2022). PubMed: 35562866
- Gomes AP et al. Altered propionate metabolism contributes to tumour progression and aggressiveness. Nat Metab 4:435-443 (2022). PubMed: 35361954
- Lim KH et al. Antisense oligonucleotide modulation of non-productive alternative splicing upregulates gene expression. Nat Commun 11:3501 (2020). PubMed: 32647108