Anti-PCCA 抗体 (ab154254)
Key features and details
- Rabbit polyclonal to PCCA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PCCA antibody
PCCA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PCCA -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment, corresponding to a region within amino acids 482-716 of Human PCCA (UniProt ID: P05165).
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ポジティブ･コントロール
- HeLa whole cell lysate (ab150035); U87 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154254の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
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| IHC-P |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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パスウェイ
Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3. -
関連疾患
Defects in PCCA are the cause of propionic acidemia type I (PA-1) [MIM:606054]. PA-1 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein. -
配列類似性
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 5095 Human
- Entrez Gene: 110821 Mouse
- Entrez Gene: 687008 Rat
- Omim: 232000 Human
- SwissProt: P05165 Human
- SwissProt: Q91ZA3 Mouse
- SwissProt: P14882 Rat
- Unigene: 80741 Human
see all -
別名
- mitochondrial antibody
- Pcca antibody
- pccA COMPLEMENTATION GROUP antibody
see all
画像
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Western blot - Anti-PCCA antibody (ab154254)Anti-PCCA antibody (ab154254) at 1/5000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 77 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCA antibody (ab154254)Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling PCCA with ab154254 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154254 は 1 報の論文で使用されています。
- Zeng ZG et al. Therapeutic potential of living donor liver transplantation from heterozygous carrier donors in children with propionic acidemia. Orphanet J Rare Dis 17:62 (2022). PubMed: 35189944