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リコンビナントRabMAb

Anti-PCBP2/hnRNP E2 抗体 [EPR14858] (ab184962)

Submit a review Submit a question References (7)
Western blot - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
  • Immunocytochemistry/ Immunofluorescence - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
  • Flow Cytometry (Intracellular) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
  • Immunoprecipitation - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
  • Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14858] to PCBP2/hnRNP E2
  • Suitable for: IP, ICC/IF, WB, IHC-P, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-PCBP2/hnRNP E2 antibody [EPR14858]
    PCBP2/hnRNP E2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14858] to PCBP2/hnRNP E2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, Raji and K562 cell lysates; Human clear cell carcinoma of kidney tissue; Human skin tissue; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184962の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/40 - 1/60.
ICC/IF
1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 35, 39 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/110.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
IP
1/40 - 1/60.
ICC/IF
1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 35, 39 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/110.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.

  • 組織特異性

    Detected in all tissues examined.

  • 配列類似性

    Contains 3 KH domains.

  • 翻訳後修飾

    Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.

  • 細胞内局在

    Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Alpha CP2 antibody
      • Alpha-CP2 antibody
      • alphaCP-2 antibody
      • Cbp antibody
      • CTBP antibody
      • Heterogeneous nuclear ribonucleoprotein E2 antibody
      • Heterogenous nuclear ribonucleoprotein E2 antibody
      • hnRNP E2 antibody
      • hnRNP-E2 antibody
      • HNRNPE2 antibody
      • Hnrnpx antibody
      • HNRPE2 antibody
      • Hnrpx antibody
      • MGC110998 antibody
      • PCBP2 antibody
      • PCBP2_HUMAN antibody
      • poly(rC) binding protein 2 antibody
      • Poly(rC)-binding protein 2 antibody
      • Putative heterogeneous nuclear ribonucleoprotein X antibody
      • rCbinding protein 2 antibody
      see all

    画像

    • Western blot - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
      Western blot - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
      All lanes : Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : K562 cell lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)

      Immunohistochemical analysis of paraffin-embedded human skin tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)

      Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling PCBP2/hnRNP E2 with ab184962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
      Immunocytochemistry/ Immunofluorescence - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)

      Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling PCBP2/hnRNP E2 with ab184962 at 1/250 dilution (red) followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    • Flow Cytometry (Intracellular) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
      Flow Cytometry (Intracellular) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)

      Intracellular flow cytometric anlaysis of HeLa cells (2% paraformaldehyde-fixed)labeling PCBP2/hnRNP E2 with ab184962 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

       

       

    • Immunoprecipitation - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
      Immunoprecipitation - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)

      Immunoprecipitation analysis of HeLa lysates labeling PCBP2/hnRNP E2 with ab18492 at 1/50 dilution (Lane 1) and PBS (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used as secondary antibody. 

    • Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)
      Anti-PCBP2/hnRNP E2 antibody [EPR14858] (ab184962)

    参考文献 (7)

    ab184962 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184962 は 7 報の論文で使用されています。

    • Guo S  et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12:42 (2021). PubMed: 33414446
    • Xing Z  et al. CircSERPINA3 regulates SERPINA3-mediated apoptosis, autophagy and aerobic glycolysis of prostate cancer cells by competitively binding to MiR-653-5p and recruiting BUD13. J Transl Med 19:492 (2021). PubMed: 34861864
    • Ghosh G  et al. Poly C Binding Protein 2 dependent nuclear retention of the utrophin-A mRNA in C2C12 cells. RNA Biol 18:612-622 (2021). PubMed: 34904931
    • Bae DH  et al. Acireductone dioxygenase 1 (ADI1) is regulated by cellular iron by a mechanism involving the iron chaperone, PCBP1, with PCBP2 acting as a potential co-chaperone. Biochim Biophys Acta Mol Basis Dis N/A:165844 (2020). PubMed: 32480040
    • Liu Y  et al. CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer. Cell Death Dis 10:644 (2019). PubMed: 31501420
    • Malik N  et al. The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription. Nat Commun 10:2071 (2019). PubMed: 31061501
    • Booy EP  et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). PubMed: 30247708

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