Anti-PCBP2/hnRNP E2 抗体 (ab154245)
Key features and details
- Rabbit polyclonal to PCBP2/hnRNP E2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PCBP2/hnRNP E2 antibody
PCBP2/hnRNP E2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PCBP2/hnRNP E2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 85-365 of Human PCBP2/hnRNP E2 (Q15366).
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ポジティブ･コントロール
- Jurkat, Raji, K562 and NCI-H929 whole cell lysates. HeLa cells. Human muscle tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154245の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0 |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation. -
組織特異性
Detected in all tissues examined. -
配列類似性
Contains 3 KH domains. -
翻訳後修飾
Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity. -
細胞内局在
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5094 Human
- Omim: 601210 Human
- SwissProt: Q15366 Human
- Unigene: 546271 Human
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別名
- Alpha CP2 antibody
- Alpha-CP2 antibody
- alphaCP-2 antibody
see all
画像
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Western blot - Anti-PCBP2/hnRNP E2 antibody (ab154245)All lanes : Anti-PCBP2/hnRNP E2 antibody (ab154245) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : NCI H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PCBP2/hnRNP E2 antibody (ab154245)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling PCBP2/hnRNP E2 with ab154245 at 1/500. Lower panel shows costaining with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody (ab154245)Immunohistochemical analysis of paraffin embedded HeLa xenograft tissue labeling PCBP2/hnRNP E2 with ab154245 at 1/500. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody (ab154245)Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling PCBP2/hnRNP E2 with ab154245 at 1/500.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154245 は論文での使用が確認できていません。