Anti-PCBP1 抗体 [EPR11055] (ab168378)
![Western blot - Anti-PCBP1 antibody [EPR11055] (ab168378)](https://www.abcam.co.jp/ps/products/168/ab168378/Images/ab168378-478731-anti-pcbp1-antibody-epr11055-westernblot-human.jpg "Western blot - Anti-PCBP1 antibody [EPR11055] (ab168378)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11055] to PCBP1
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PCBP1 antibody [EPR11055]
PCBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11055] to PCBP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human PCBP1.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11055 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168378の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 37 kDa.
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| IP |
1/10 - 1/100.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 37 kDa. |
|
IP
1/10 - 1/100. |
ターゲット情報
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機能
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. -
組織特異性
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands. -
配列類似性
Contains 3 KH domains. -
翻訳後修飾
Phosphorylated; lowers poly(rC)-binding activity. -
細胞内局在
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5093 Human
- Omim: 601209 Human
- SwissProt: Q15365 Human
- Unigene: 2853 Human
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別名
- Alpha-CP1 antibody
- Heterogeneous nuclear ribonucleoprotein E1 antibody
- heterogenous nuclear ribonucleoprotein E1 antibody
see all
画像
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Western blot - Anti-PCBP1 antibody [EPR11055] (ab168378)All lanes : Anti-PCBP1 antibody [EPR11055] (ab168378) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa -
![Immunoprecipitation - Anti-PCBP1 antibody [EPR11055] (ab168378)](https://www.abcam.co.jp/ps/products/168/ab168378/Images/ab168378-6-anti-pcbp1-antibody-epr11055-immunoprecipitation-293t-human.jpg)
Immunoprecipitation - Anti-PCBP1 antibody [EPR11055] (ab168378)Purified ab168378 at 1:50 dilution (2µg) immunoprecipitating PCBP1 in 293T whole cell lysate.
Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10 µg.
Lane 2 (+): ab168378 + 293T whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab168378 in 293T whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: kDa -
![Anti-PCBP1 antibody [EPR11055] (ab168378)](https://www.abcam.co.jp/ps/products/168/ab168378/Images/ab168378-3-benefits-of-recombinant-antibodies.png)
Anti-PCBP1 antibody [EPR11055] (ab168378)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab168378 は 5 報の論文で使用されています。
- Cousineau SE et al. Poly(rC)-Binding Protein 1 Limits Hepatitis C Virus Virion Assembly and Secretion. Viruses 14:N/A (2022). PubMed: 35215884
- Jayavelu AK et al. Splicing factor YBX1 mediates persistence of JAK2-mutated neoplasms. Nature 588:157-163 (2020). PubMed: 33239784
- Bae DH et al. Acireductone dioxygenase 1 (ADI1) is regulated by cellular iron by a mechanism involving the iron chaperone, PCBP1, with PCBP2 acting as a potential co-chaperone. Biochim Biophys Acta Mol Basis Dis N/A:165844 (2020). PubMed: 32480040
- Rizzotto D et al. Nutlin-Induced Apoptosis Is Specified by a Translation Program Regulated by PCBP2 and DHX30. Cell Rep 30:4355-4369.e6 (2020). PubMed: 32234473
- Ghanem LR et al. The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development. Mol Cell Biol 36:304-19 (2016). PubMed: 26527618