Anti-PCB 抗体 (ab115579)
Key features and details
- Goat polyclonal to PCB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PCB antibody
PCB 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PCB -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Dog, Pig
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免疫原
Synthetic peptide:
KFKEVKKAYVEANQ
with a Cysteine residue linker, corresponding to internal sequence amino acids 886-899 of Human PCB (NP_000911.2). -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115579の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.03 - 1 µg/ml. Predicted molecular weight: 130 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.03 - 1 µg/ml. Predicted molecular weight: 130 kDa. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. -
パスウェイ
Carbohydrate biosynthesis; gluconeogenesis. -
関連疾患
Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia. -
配列類似性
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 338471 Cow
- Entrez Gene: 5091 Human
- Entrez Gene: 18563 Mouse
- Entrez Gene: 397630 Pig
- Entrez Gene: 25104 Rat
- Omim: 608786 Human
- SwissProt: Q29RK2 Cow
- SwissProt: P11498 Human
see all -
別名
- mitochondrial antibody
- OTTHUMP00000235155 antibody
- OTTHUMP00000235156 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody (ab115579)ab115579, at 5µg/ml, staining PCB in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Western blot - Anti-PCB antibody (ab115579)Anti-PCB antibody (ab115579) at 0.03 µg/ml + Mouse Liver lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 130 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab115579 は 4 報の論文で使用されています。
- Torrini C et al. Lactate is an epigenetic metabolite that drives survival in model systems of glioblastoma. Mol Cell 82:3061-3076.e6 (2022). PubMed: 35948010
- Li X et al. Probiotics Ameliorate Colon Epithelial Injury Induced by Ambient Ultrafine Particles Exposure. Adv Sci (Weinh) 6:1900972 (2019). PubMed: 31559135
- Gao L et al. Gene expression analyses reveal metabolic specifications in acute O2 -sensing chemoreceptor cells. J Physiol 595:6091-6120 (2017). PubMed: 28718507
- Lussey-Lepoutre C et al. Loss of succinate dehydrogenase activity results in dependency on pyruvate carboxylation for cellular anabolism. Nat Commun 6:8784 (2015). IHC . PubMed: 26522426