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リコンビナントRabMAb

Anti-PC4 抗体 [EPR9281] (ab154852)

Submit a review Submit a question References (1)
Western blot - Anti-PC4 antibody [EPR9281] (ab154852)
  • Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)
  • Immunoprecipitation - Anti-PC4 antibody [EPR9281] (ab154852)
  • Western blot - Anti-PC4 antibody [EPR9281] (ab154852)
  • Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)
  • Anti-PC4 antibody [EPR9281] (ab154852)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9281] to PC4
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PC4 antibody [EPR9281]
    PC4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR9281] to PC4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IPmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human PC4 aa 1-100. The exact sequence is proprietary.
    Database link: P53999

  • ポジティブ･コントロール

    • MCF7, T47D, Jurkat and PC3 cell lysates; MCF7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR9281

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154852の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
ICC/IF
1/100 - 1/250.

For unpurified, use 1/100 - 1/250.
IP
1/10 - 1/100.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
ICC/IF
1/100 - 1/250.

For unpurified, use 1/100 - 1/250.
IP
1/10 - 1/100.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).

  • 配列類似性

    Belongs to the transcriptional coactivator PC4 family.

  • 翻訳後修飾

    Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Activated RNA polymerase II transcriptional coactivator p15 antibody
      • Interferon related developmental regulator 1 antibody
      • MGC102747 antibody
      • p14 antibody
      • P15 antibody
      • PC4 antibody
      • PC4 LSB antibody
      • Positive cofactor 4 antibody
      • RPO2TC1 antibody
      • Sub1 antibody
      • SUB1 homolog antibody
      • TCP4_HUMAN antibody
      see all

    画像

    • Western blot - Anti-PC4 antibody [EPR9281] (ab154852)
      Western blot - Anti-PC4 antibody [EPR9281] (ab154852)
      All lanes : Anti-PC4 antibody [EPR9281] (ab154852) at 1/5000 dilution (purified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : T-47D cell lysate
      Lane 3 : PC-3 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)
      Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)

      Immunofluorescence staining of T47D cells with purified ab154852 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab154852 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Immunoprecipitation - Anti-PC4 antibody [EPR9281] (ab154852)
      Immunoprecipitation - Anti-PC4 antibody [EPR9281] (ab154852)
      ab154852 (purified) at 1/70 immunoprecipitating PC4 in 10 μg Jurkat (Lanes 1 and 2, observed at 14 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • Western blot - Anti-PC4 antibody [EPR9281] (ab154852)
      Western blot - Anti-PC4 antibody [EPR9281] (ab154852)
      All lanes : Anti-PC4 antibody [EPR9281] (ab154852) at 1/1000 dilution (unpurified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : T47D cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 14 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)
      Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)

      Immunofluorescent analysis of MCF7 cells labeling PC4 with unpurified ab154852 at 1/100.

    • Anti-PC4 antibody [EPR9281] (ab154852)
      Anti-PC4 antibody [EPR9281] (ab154852)

    参考文献 (1)

    ab154852 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab154852 は 1 報の論文で使用されています。

    • Liu G  et al. ARID1B/SUB1-activated lncRNA HOXA-AS2 drives the malignant behaviour of hepatoblastoma through regulation of HOXA3. J Cell Mol Med 25:3524-3536 (2021). PubMed: 33683826

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