Anti-PC4 抗体 (ab84459)
Key features and details
- Rabbit polyclonal to PC4
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PC4 antibody
PC4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PC4 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide corresponding to a region between residues 77 and 127 of Human PC4 (NP_006704.2)
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ポジティブ･コントロール
- Human: bladder carcinoma, breast carcinoma, colon carcinoma, linitis plastica stomach cancer, ovarian carcinoma, pancreatic islet cell tumor, prostate carcinoma, skin basal cell carcinoma, skin melanoma, skin squamous cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma. Mouse: skin, seratoma
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84459の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
| 特記事項 |
|---|
追加情報
IHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer may improve staining.
Note: likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Note: likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA). -
配列類似性
Belongs to the transcriptional coactivator PC4 family. -
翻訳後修飾
Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10923 Human
- Entrez Gene: 20024 Mouse
- Entrez Gene: 192269 Rat
- Omim: 600503 Human
- SwissProt: P53999 Human
- SwissProt: P11031 Mouse
- SwissProt: Q63396 Rat
- Unigene: 229641 Human
see all -
別名
- Activated RNA polymerase II transcriptional coactivator p15 antibody
- Interferon related developmental regulator 1 antibody
- MGC102747 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC4 antibody (ab84459)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue using 1/250 ab84459, followed by DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC4 antibody (ab84459)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue using 1/250 ab84459, followed by DAB staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC4 antibody (ab84459)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse teratoma tissue using 1/250 ab84459, followed by DAB staining.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab84459 は 4 報の論文で使用されています。
- Ochiai K et al. Protocol for in vitro BCR-mediated plasma cell differentiation and purification of chromatin-associated proteins. STAR Protoc 2:100633 (2021). PubMed: 34258594
- Liao F et al. PC4 serves as a negative regulator of skin wound healing in mice. Burns Trauma 8:tkaa010 (2020). PubMed: 32373645
- Ochiai K et al. Chromatin Protein PC4 Orchestrates B Cell Differentiation by Collaborating with IKAROS and IRF4. Cell Rep 33:108517 (2020). PubMed: 33357426
- Westphal N et al. Nuclear fragments of the neural cell adhesion molecule NCAM with or without polysialic acid differentially regulate gene expression. Sci Rep 7:13631 (2017). PubMed: 29051583