Anti-PBRM1/BAF180 抗体 (ab245399)
Key features and details
- Rabbit polyclonal to PBRM1/BAF180
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PBRM1/BAF180 antibody
PBRM1/BAF180 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PBRM1/BAF180 -
由来種
Rabbit -
アプリケーション
適用あり: IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide within Human PBRM1/BAF180 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. AAP34197.1
Database link: Q86U86 -
ポジティブ･コントロール
- IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245399 was affinity purified using an epitope specific to PBRM1/BAF180 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245399の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Act as a negative regulator of cell proliferation. -
組織特異性
Widely expressed. -
関連疾患
Defects in PBRM1 are a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. -
配列類似性
Contains 2 BAH domains.
Contains 6 bromo domains.
Contains 1 HMG box DNA-binding domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 55193 Human
- Entrez Gene: 66923 Mouse
- Omim: 606083 Human
- SwissProt: Q86U86 Human
- SwissProt: Q8BSQ9 Mouse
- Unigene: 189920 Human
- Unigene: 476320 Human
- Unigene: 27913 Mouse
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別名
- BAF180 antibody
- BRG1-associated factor 180 antibody
- CG11375 antibody
see all
画像
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Immunoprecipitation - Anti-PBRM1/BAF180 antibody (ab245399)
PBRM1/BAF180 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245399 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using a different Anti-PBRM1/BAF180 antibody at a 1/1000 dilution.
Lane 1: ab245399, Lot 3 IP in HeLa whole cell lysate.
Lane 2: ab245399, Lot 2 IP in HeLa whole cell lysate.
Lane 3: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
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Immunoprecipitation - Anti-PBRM1/BAF180 antibody (ab245399)PBRM1/BAF180 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245399 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using a different Anti-PBRM1/BAF180 antibody at 1 µg/mL.
Lane 1: ab245399 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared in NETN buffer.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab245399 は 1 報の論文で使用されています。
- Huang K et al. A novel EZH2 inhibitor induces synthetic lethality and apoptosis in PBRM1-deficient cancer cells. Cell Cycle 19:758-771 (2020). PubMed: 32093567