AB242318

Anti-PBRM1/BAF180 抗体 [CL0331]

Name: Anti-PBRM1/BAF180 抗体 [CL0331] (ab242318) | Abcam
Brand: Abcam Limited
SKU: AB242318
Price: 90000 JPY
Availability: InStock

Anti-PBRM1/BAF180 antibody [CL0331]

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal PBRM1/BAF180 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PBRM1 aa 100-250.

別名を表示する

BAF180, PB1, PBRM1, Protein polybromo-1, hPB1, BRG1-associated factor 180, Polybromo-1D

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

Paraffin-embedded human breast cancer tissue stained for PBRM1/BAF180 using ab242318 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

Negative Control :

Paraffin-embedded human skeletal muscle tissue stained for PBRM1/BAF180 using ab242318 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

Paraffin-embedded human endometrium tissue stained for PBRM1/BAF180 using ab242318 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

Paraffin-embedded human kidney cancer tissue stained for PBRM1/BAF180 using ab242318 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

All lanes:

Western blot - Anti-PBRM1/BAF180 antibody [CL0331] (ab242318) at 1 µg/mL

Lane 1:

RT-4 cells transfected with control siRNA

Lane 2:

RT-4 cells transfected with target specific siRNA probe

Predicted band size: 193 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

CL0331

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human PBRM1 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q86U86

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PBRM1 also known as BAF180 is an important component of the SWI/SNF chromatin remodeling complex. It has a molecular weight of approximately 187 kDa. Within the cell PBRM1 is expressed in various tissues but shows higher expression in kidney and breast tissues. Its role involves binding to acetylated histones which influences chromatin structure and gene expression regulation.

Biological function summary

The PBRM1/BAF180 protein functions as part of the SWI/SNF complex which includes the catalytic subunit BRG1 or BRM. This complex is important for altering chromatin architecture which regulates access of transcriptional machinery to DNA. It plays a significant role in the control of cell growth and differentiation processes. The structural integrity of PBRM1 is necessary for the functionality of the SWI/SNF complex.

Pathways

PBRM1/BAF180 is an important player in pathways involving chromatin remodeling and transcriptional regulation. It is implicated in the WNT signaling pathway interacting with proteins such as β-catenin to influence gene transcription. It also partakes in the p53 pathway where it contributes to the regulation of cell cycle and apoptosis by modulating p53 activity.

Mutations in PBRM1 are frequently observed in renal cell carcinoma. Its disruption affects chromatin remodeling which disrupts normal gene expression contributing to cancer progression. PBRM1 also relates to breast cancer where alterations can promote tumor formation. In these contexts the interaction of PBRM1 with other proteins like VHL in renal carcinoma helps elucidate its role in tumor suppression and development.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation.

See full target information PBRM1

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 55:e13190 PubMed35102634

2022

Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system.

Applications

Unspecified application

Species

Unspecified reactive species

Thao Thi Thanh Ngo,Bella Rossbach,Isabelle Sébastien,Julia C Neubauer,Andreas Kurtz,Krithika Hariharan

View all publications

style

left-column

style

left-column

style

right-column