AB245399

Anti-PBRM1/BAF180 抗体

Name: Anti-PBRM1/BAF180 抗体 (ab245399) | Abcam
Brand: Abcam Limited
SKU: AB245399
Price: 75000 JPY
Availability: InStock

Anti-PBRM1/BAF180 antibody

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(2 Publications)

Rabbit Polyclonal PBRM1/BAF180 antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PBRM1 aa 1-50.

別名を表示する

BAF180, PB1, PBRM1, Protein polybromo-1, hPB1, BRG1-associated factor 180, Polybromo-1D

2 Images

Supplier Data

Immunoprecipitation - Anti-PBRM1/BAF180 antibody (AB245399)

PBRM1/BAF180 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245399 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using a different Anti-PBRM1/BAF180 antibody at a 1/1000 dilution.

Lane 1 : ab245399, Lot 3 IP in HeLa whole cell lysate.

Lane 2 : ab245399, Lot 2 IP in HeLa whole cell lysate.

Lane 3 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-PBRM1/BAF180 antibody (ab245399)

Predicted band size: 193 kDa

false

Supplier Data

Immunoprecipitation - Anti-PBRM1/BAF180 antibody (AB245399)

Lane 1 : ab245399 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-PBRM1/BAF180 antibody (ab245399)

Predicted band size: 193 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human PBRM1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q86U86

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab245399 was affinity purified using an epitope specific to Baf180 immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PBRM1 also known as BAF180 is an important component of the SWI/SNF chromatin remodeling complex. It has a molecular weight of approximately 187 kDa. Within the cell PBRM1 is expressed in various tissues but shows higher expression in kidney and breast tissues. Its role involves binding to acetylated histones which influences chromatin structure and gene expression regulation.

Biological function summary

The PBRM1/BAF180 protein functions as part of the SWI/SNF complex which includes the catalytic subunit BRG1 or BRM. This complex is important for altering chromatin architecture which regulates access of transcriptional machinery to DNA. It plays a significant role in the control of cell growth and differentiation processes. The structural integrity of PBRM1 is necessary for the functionality of the SWI/SNF complex.

Pathways

PBRM1/BAF180 is an important player in pathways involving chromatin remodeling and transcriptional regulation. It is implicated in the WNT signaling pathway interacting with proteins such as β-catenin to influence gene transcription. It also partakes in the p53 pathway where it contributes to the regulation of cell cycle and apoptosis by modulating p53 activity.

Mutations in PBRM1 are frequently observed in renal cell carcinoma. Its disruption affects chromatin remodeling which disrupts normal gene expression contributing to cancer progression. PBRM1 also relates to breast cancer where alterations can promote tumor formation. In these contexts the interaction of PBRM1 with other proteins like VHL in renal carcinoma helps elucidate its role in tumor suppression and development.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation.

See full target information PBRM1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 56:564-575 PubMed38449391

2024

The deubiquitinase BRCC3 increases the stability of ZEB1 and promotes the proliferation and metastasis of triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qidi Huang,Shurong Zheng,Huayan Gu,Zhi Yang,Yiqiao Lu,Xia Yu,Guilong Guo

Cell cycle (Georgetown, Tex.) 19:758-771 PubMed32093567

2020

A novel EZH2 inhibitor induces synthetic lethality and apoptosis in PBRM1-deficient cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kejia Huang,Rong Sun,Jiarong Chen,Qianye Yang,Yucheng Wang,Yang Zhang,Kun Xie,Tiantian Zhang,Rui Li,Qi Zhao,Liang Zou,Jian Li

View all publications

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