Anti-PBR 抗体 (ab154878)
Key features and details
- Rabbit polyclonal to PBR
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
製品の概要
-
製品名
Anti-PBR antibody
PBR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PBR -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Horse, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
-
免疫原
Synthetic peptide within Rat PBR aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P16257 -
ポジティブ･コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Normal Rat Testes
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154878の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. -
組織特異性
Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids. -
配列類似性
Belongs to the TspO/BZRP family. -
細胞内局在
Mitochondrion membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 706 Human
- Entrez Gene: 12257 Mouse
- Entrez Gene: 24230 Rat
- Omim: 109610 Human
- SwissProt: B1AH88 Human
- SwissProt: P30536 Human
- SwissProt: P50637 Mouse
- SwissProt: P16257 Rat
see all -
別名
- Benzodiazapine receptor (peripheral) antibody
- Benzodiazepine peripheral binding site antibody
- BPBS antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBR antibody (ab154878)
IHC image of TPBR staining in normal Rat testes formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154878, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab154878 は 6 報の論文で使用されています。
- Kitade M et al. Evaluation of (R)-[11C]PK11195 PET/MRI for Spinal Cord-Related Neuropathic Pain in Patients with Cervical Spinal Disorders. J Clin Med 12:N/A (2022). PubMed: 36614916
- Zhu Y et al. The Anti-Inflammatory Effect of Preventive Intervention with Ketogenic Diet Mediated by the Histone Acetylation of mGluR5 Promotor Region in Rat Parkinson's Disease Model: A Dual-Tracer PET Study. Parkinsons Dis 2022:3506213 (2022). PubMed: 36105302
- Al-Khishman NU et al. TSPO PET detects acute neuroinflammation but not diffuse chronically activated MHCII microglia in the rat. EJNMMI Res 10:113 (2020). PubMed: 32990808
- Koganti PP & Selvaraj V Lack of adrenal TSPO/PBR expression in hamsters reinforces correlation to triglyceride metabolism. J Endocrinol 247:1-10 (2020). PubMed: 32698131
- Huang S et al. Monitoring?the?Progression?of?Chronic Liver Damage in Rats Using [18F]PBR06. Mol Imaging Biol 21:669-675 (2019). PubMed: 30280292
- Owen DR et al. TSPO mutations in rats and a human polymorphism impair the rate of steroid synthesis. Biochem J 474:3985-3999 (2017). WB ; Rat . PubMed: 29074640