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リコンビナントRabMAb

Anti-PBK/SPK (phospho T9) 抗体 [EPR2475(N)] (ab184953)

Submit a review Submit a question References (2)
Western blot - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
  • Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
  • Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
  • Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2475(N)] to PBK/SPK (phospho T9)
  • Suitable for: Flow Cyt (Intra), IHC-P, WB
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)]
    PBK/SPK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2475(N)] to PBK/SPK (phospho T9)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-P, WBmore details

  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa cells/lysate treated with Nocodazole. IHC-P: Human testis and rat spleen tissues. Flow Cyt (intra): HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184953の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/800.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
特記事項
Flow Cyt (Intra)
1/800.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).

ターゲット情報

  • 機能

    Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

  • 組織特異性

    Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.

  • 配列類似性

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylated; in a cell-cycle dependent manner at mitosis.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cancer/testis antigen 84 antibody
      • CT84 antibody
      • Epididymis luminal protein 164 antibody
      • FLJ14385 antibody
      • HEL164 antibody
      • Lymphokine activated killer T cell originated protein kinase antibody
      • Lymphokine-activated killer T-cell-originated protein kinase antibody
      • MAPKK like protein kinase antibody
      • MAPKK-like protein kinase antibody
      • Nori 3 antibody
      • Nori-3 antibody
      • Nori3 antibody
      • PBK antibody
      • PDZ binding kinase antibody
      • PDZ-binding kinase antibody
      • Serine/threonine protein kinase antibody
      • Spermatogenesis related protein kinase antibody
      • Spermatogenesis-related protein kinase antibody
      • SPK antibody
      • T LAK cell originated protein kinase antibody
      • T-LAK cell-originated protein kinase antibody
      • TOPK antibody
      • TOPK_HUMAN antibody
      see all

    画像

    • Western blot - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
      Western blot - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
      All lanes : Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953) at 1/5000 dilution

      Lane 1 : HeLa cell lysate treated with Nocodazole
      Lane 2 : HeLa cell lysate untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 36 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?

    • Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
      Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

      Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilised HeLa (Human cervix adenocarcinoma epithelial cell) treated with 0.1µg/mL Nocodazole for 24h (Red) / Untreated control (Green) cells labeling PBK/SPK (phospho T9) with ab184953 at 1/800 dilution compared with a Isotype control Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.Higher expression in part of Nocodazole treated samples (mitotic cell induction) which is consistent with cell cycle Flow Cytometry data. 

       

       

    • Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
      Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PBK/SPK (phospho T9) with ab184953 at 1/800 dilution (Right) compared with aRabbit monoclonal IgG Isotype control (ab172730) (Left). Cells permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.Highest expression at M phase. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

      Immunohistochemical analysis of paraffin-embedded human testis tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

      Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
      Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

    参考文献 (2)

    ab184953 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184953 は 2 報の論文で使用されています。

    • Shu H  et al. The role of the SGK3/TOPK signaling pathway in the transition from acute kidney injury to chronic kidney disease. Front Pharmacol 14:1169054 (2023). PubMed: 37361201
    • Pirovano G  et al. TOPK modulates tumour-specific radiosensitivity and correlates with recurrence after prostate radiotherapy. Br J Cancer 117:503-512 (2017). WB ; Human . PubMed: 28677687

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