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AB76235

Anti-Paxillin (phospho Y181) 抗体 [EP41(2)Y]

Anti-Paxillin (phospho Y181) antibody [EP41(2)Y]

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(2 Publications)

Rabbit Recombinant Monoclonal Paxillin phospho Y181 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

別名を表示する

Paxillin, PXN

2 Images
Western blot - Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (AB76235)
  • WB

Supplier Data

Western blot - Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (AB76235)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235) at 1/10000 dilution

Lane 1:

Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa treated with 1mM pervanadate for 30min whole cell lysate at 15 µg

Lane 3:

HeLa treated with 1mM pervanadate for 30min whole cell lysate. Then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 65 kDa

Observed band size: 68 kDa

false

Western blot - Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (AB76235)
  • WB

Unknown

Western blot - Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (AB76235)

All lanes:

Western blot - Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with pervanadate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 65 kDa

Observed band size: 68 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP41(2)Y

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

This product has switched from a hybridoma to recombinant production method on 20th November 2023.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Paxillin also known as PXN is a cytoskeletal protein involved in the assembly of focal adhesions. It has a molecular weight of approximately 68 kDa. You will find Paxillin expressed widely in various tissues including the liver colon and lung. Paxillin plays a part in cell motility by interacting with other focal adhesion proteins. It plays a significant mechanical role by serving as a docking site for structural and signaling molecules.
Biological function summary

Paxillin serves key functions in cellular processes such as migration proliferation and survival. It interacts with different proteins including vinculin and talin forming part of a complex at the focal adhesion sites. The phosphorylation state of Paxillin can modulate its interactions and in this way influence the assembly of signaling complexes that control dynamic cellular processes.

Pathways

Paxillin participates in the integrin signaling and MAPK pathways. It operates by linking integrin receptors to the actin cytoskeleton facilitating signal transduction. Paxillin phosphorylation is an important regulatory mechanism within these pathways. In particular its interaction with focal adhesion kinase (FAK) and Src family kinases signifies its role in transmitting signals from the extracellular matrix to the cellular interior which impacts cell behavior and response.

Paxillin has links with osteosarcoma and lung cancer. Deregulation of Paxillin expression or its phosphorylation state contributes to oncogenic transformation and tumor progression. In cancer Paxillin associates with altered activity of proteins such as Src and FAK exacerbating disease states. This relationship highlights Paxillin's potential as a biomarker and therapeutic target in treating related malignancies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.
See full target information PXN pY181
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer research : MCR 12:440-6 PubMed24337068

2013

Actin-binding protein, Espin: a novel metastatic regulator for melanoma.

Applications

WB

Species

Mouse

Takeshi Yanagishita,Ichiro Yajima,Mayuko Kumasaka,Yoshiyuki Kawamoto,Toyonori Tsuzuki,Yoshinari Matsumoto,Daisuke Watanabe,Masashi Kato

PloS one 6:e25103 PubMed21966428

2011

Galectin-3 facilitates cell motility in gastric cancer by up-regulating protease-activated receptor-1 (PAR-1) and matrix metalloproteinase-1 (MMP-1).

Applications

WB

Species

Human

Seok-Jun Kim,Ji-Young Shin,Kang-Duck Lee,Young-Ki Bae,Il-Ju Choi,Seok Hee Park,Kyung-Hee Chun
View all publications
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代替製品

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Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547)

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Primary Antibodies

AB32115

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  • 0
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Primary Antibodies

AB216652

Anti-Paxillin antibody [Y113] - BSA and Azide free

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  • 0
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Primary Antibodies

AB238950

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  • 0
  • 0
Primary Antibodies

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BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

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  • 5
  • 21

組合せ製品

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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  • 5
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Primary Antibodies

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Anti-Vinculin antibody [EPR8185] - Loading Control

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Primary Antibodies

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Anti-Vitronectin/S-Protein antibody [EP873Y]

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  • 0
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Abcam product promise

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