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Anti-Paxillin (phospho S83) 抗体 (ab31833)

Submit a review Q&A (1)
Western blot - Anti-Paxillin (phospho S83) antibody (ab31833)

    Key features and details

    • Rabbit polyclonal to Paxillin (phospho S83)
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Paxillin (phospho S83) antibody
      Paxillin 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Paxillin (phospho S83)

    • 由来種

      Rabbit

    • 特異性

      ab31833 detects a 68kDa protein corresponding to the molecular mass of phosphorylated paxillin.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Mouse

    • 免疫原

      Synthetic peptide corresponding to Mouse Paxillin (phospho S83) conjugated to keyhole limpet haemocyanin.
      Database link: Q8VI36

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab31833の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/1000. Predicted molecular weight: 68 kDa.

    In 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
    特記事項
    WB
    1/1000. Predicted molecular weight: 68 kDa.

    In 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.

    ターゲット情報

    • 機能

      Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

    • 配列類似性

      Belongs to the paxillin family.
      Contains 4 LIM zinc-binding domains.

    • 翻訳後修飾

      Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.

    • 細胞内局在

      Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
    • Information by UniProt

    • 参照データベース

      • 別名

        • FLJ16691 antibody
        • FLJ23042 antibody
        • Paired box protein Pax 1 antibody
        • PAX 1 antibody
        • PAX1 antibody
        • PAXI_HUMAN antibody
        • Paxillin alpha antibody
        • Paxillin antibody
        • PXN antibody
        • PXN protein antibody
        see all

      画像

      • Western blot - Anti-Paxillin (phospho S83) antibody (ab31833)
        Western blot - Anti-Paxillin (phospho S83) antibody (ab31833)
        Western blot analysis of immunoprecipitated paxillin (arrow) in mouse macrophages untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min (lanes 2, 4, 5, & 6). Lysates were immunoprecipitated with anti-Paxillin. The blots were probed with anti-Paxillin or anti-phospho-Paxillin (Ser-83). The latter was used in the presence of phospho-Paxillin (Ser-83) peptide (lane 5) or phospho-Paxillin (Ser-85) peptide (lane 6). Immunoglobulin (Ig) chains from the immunoprecipitation were observed above and below the paxillin bands.

      参考文献 (0)

      ab31833 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab31833 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Question

      Does the Paxillin (phospho S83) antibody (ab31833) react against human or monkey lysates? I would like to test for phosphorylation of the equivalent residue in cell lines of these organisms and can't find a human or monkey specific antibody. As such, I would be willing to test whether or not this antibody would react with phosphorylated money or human paxillin. Do you cell the positive control lysates (mouse macrophages treated with Calyculin A?) Your help would be appreciated.

      Read More
      Answer

      Thank you for your enquiry. This antibody has yet to be tested in human or monkey tissue. The conserved site in human paxillin (Ser-83) has high homology with the mouse paxillin (Ser-83). There is only 2 amino acid differences, however there is evidence that in human the phosphorylation site is Ser-85 rather than Ser-83 (Journal of Cell Biology, (2004) Vol 164(4):593-602.). We have only shown that ab31833 works in mouse, but it may be useful for detecting Ser-83 in human. We currently do not sell the positive control I'm afraid. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If the antibody is being used in an untested species or application, we cannot offer a replacement or refund; however, we invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for discounts on future antibody purchases or rewards such as Amazon.com gift certificates. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

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