Anti-Paxillin 抗体 [M107] (ab23510)
Key features and details
- Mouse monoclonal [M107] to Paxillin
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Paxillin antibody [M107]
Paxillin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [M107] to Paxillin -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein (Human).
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ポジティブ・コントロール
- A431 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
M107 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23510の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 65 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 65 kDa). |
ターゲット情報
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機能
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). -
配列類似性
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains. -
翻訳後修飾
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. -
細胞内局在
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. - Information by UniProt
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参照データベース
- Entrez Gene: 5829 Human
- Omim: 602505 Human
- SwissProt: P49023 Human
- Unigene: 446336 Human
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別名
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
see all
画像
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All lanes : Anti-Paxillin antibody [M107] (ab23510) at 1/1000 dilution
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : PXN knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : PC3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 65 kDaLanes 1 - 4: Merged signal (red and green). Green - ab23510 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab23510 was shown to recognize PXN in wild-type A431 cells as signal was lost at the expected MW in PXN knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PXN knockout samples were subjected to SDS-PAGE. Ab23510 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Lanes 1-2 : Anti-Paxillin antibody [M107] (ab23510)
Lanes 3-4 : Anti-Paxillin (Ser178) antibody
Lanes 1 & 3 : A431 cells (20 ug/lane) serum starved overnight
Lanes 2 & 4 : A431 cells (20 ug/lane) serum treated with EGF (100 ng/ml) for 5 min.
Predicted band size: 65 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab23510 は 4 報の論文で使用されています。
- Al-Fahad D et al. Kinetic Changes of Ptdins (3,4,5) P3 within Fast and Slow Turnover Rates of Focal Adhesion. Rep Biochem Mol Biol 11:262-269 (2022). PubMed: 36164635
- Peng J et al. Targeted Lipid Nanoparticles Encapsulating Dihydroartemisinin and Chloroquine Phosphate for Suppressing the Proliferation and Liver Metastasis of Colorectal Cancer. Front Pharmacol 12:720777 (2021). PubMed: 34690764
- Kiepas A et al. The SHCA adapter protein cooperates with lipoma-preferred partner in the regulation of adhesion dynamics and invadopodia formation. J Biol Chem 295:10535-10559 (2020). PubMed: 32299913
- Alfahad D et al. PtdIns(4,5)P2 and PtdIns(3,4,5)P3 dynamics during focal adhesions assembly and disassembly in a cancer cell line. Turk J Biol 44:381-392 (2020). PubMed: 33402865