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リコンビナントRabMAb

Anti-PAX8 抗体 [EPR18715] - BSA and Azide free (ab246332)

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ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
  • ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
  • ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
  • Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
  • Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18715] to PAX8 - BSA and Azide free
  • Suitable for: WB, ChIC/CUT&RUN-seq, IHC-P, ICC/IF, IHC-Fr
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-PAX8 antibody [EPR18715] - BSA and Azide free
    PAX8 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR18715] to PAX8 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ChIC/CUT&RUN-seq, IHC-P, ICC/IF, IHC-Frmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human thyroid carcinoma and endometrium carcinoma tissues.ICC/IF: NIH:OVCAR-3 and SK-OV-3 cells.

  • 特記事項

    ab246332 is the carrier-free version of ab191870.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab246332の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ChIC/CUT&RUN-seq
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ChIC/CUT&RUN-seq
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.

  • 組織特異性

    Expressed in the excretory system, thyroid gland and Wilms tumors.

  • 関連疾患

    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.

  • 配列類似性

    Contains 1 paired domain.

  • 発生段階

    In developing excretory system, during thyroid differentiation and in adult thyroid.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • OTTHUMP00000158659 antibody
      • OTTHUMP00000158660 antibody
      • OTTHUMP00000203723 antibody
      • OTTHUMP00000203724 antibody
      • Paired box 8 antibody
      • Paired box gene 8 antibody
      • paired box homeotic gene 8 antibody
      • Paired box protein Pax 8 antibody
      • Paired box protein Pax-8 antibody
      • Paired domain gene 8 antibody
      • PAX 8 antibody
      • PAX8 antibody
      • PAX8_HUMAN antibody
      see all

    画像

    • ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
      ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)

      This data was developed using the same antibody clone in a different buffer formulation (ab191870). 
      ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab191870 [EPR18715]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

    • ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
      ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)

      This data was developed using the same antibody clone in a different buffer formulation (ab191870). 
      ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab191870 [EPR18715]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

    • ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
      ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)

      This data was developed using the same antibody clone in a different buffer formulation (ab191870). 
      ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab191870 [EPR18715]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

    • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
      Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleus staining on SK-OV-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab191870 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191870).

    • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
      Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH:OVCAR-3 (Human ovary adenocarcinoma cell line) cells labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleus staining on NIH:OVCAR-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab191870 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191870).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)

      Immunohistochemical analysis of paraffin-embedded Human endometrium carcinoma tissue labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of the endometrium carcinoma is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191870).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)

      Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of thyroid carcinoma is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191870).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
      Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)

      Immunohistochemistry (Frozen sections) analysis of mouse kidney tissue labelling PAX8 with ab191870 at a dilution of 1/500. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000). Nuclear staining is visible in the mouse kidney.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191870).

    • Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
      Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab246332 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab246332 は論文での使用が確認できていません。

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