Anti-PAX7 抗体 (ab34360)
Key features and details
- Rabbit polyclonal to PAX7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAX7 antibody
PAX7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAX7 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human PAX7.
Immunogen の所有権に関して (Peptide available as ab37576.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34360の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa). |
ターゲット情報
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機能
Probable transcription factor. May have a role in myogenesis. -
関連疾患
Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator. -
配列類似性
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5081 Human
- Omim: 167410 Human
- SwissProt: P23759 Human
- Unigene: 113253 Human
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別名
- FLJ37460 antibody
- HUP1 antibody
- OTTHUMP00000002534 antibody
see all
画像
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Western blot - Anti-PAX7 antibody (ab34360)All lanes : Anti-PAX7 antibody (ab34360) at 1 µg/ml
Lane 1 : Human Uterus Tissue Lysate (negative control)
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Blocking peptides at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (39)
ab34360 は 39 報の論文で使用されています。
- Maniscalco E et al. Metformin regulates myoblast differentiation through an AMPK-dependent mechanism. PLoS One 18:e0281718 (2023). PubMed: 36763621
- Elia I et al. SNAI1 is upregulated during muscle regeneration and represses FGF21 and ATF3 expression by directly binding their promoters. FASEB J 36:e22401 (2022). PubMed: 35726676
- Malatesta M et al. Satellite Cells in Skeletal Muscle of the Hibernating Dormouse, a Natural Model of Quiescence and Re-Activation: Focus on the Cell Nucleus. Cells 9:N/A (2020). PubMed: 32340154
- Guo X et al. Characterization of Functional Human Skeletal Myotubes and Neuromuscular Junction Derived-From the Same Induced Pluripotent Stem Cell Source. Bioengineering (Basel) 7:N/A (2020). PubMed: 33105732
- Al Tanoury Z et al. Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro. Development 147:N/A (2020). PubMed: 32541004