Anti-PAX7 抗体 (ab187339)
Key features and details
- Rabbit polyclonal to PAX7
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAX7 antibody
PAX7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAX7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment within Human PAX7 (internal sequence). The exact sequence is proprietary. (Purified from E. coli).
Database link: P23759 -
ポジティブ･コントロール
- ICC/IF: Mouse C2C12 cells, human HeLa and MCF-7 cells; IP: HeLa; WB: HeLa, MCF-7 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187339の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 56 kDa.
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| IP |
Use a concentration of 3 µg/ml.
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| ICC/IF | (1) |
1/20 - 1/200.
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| 特記事項 |
|---|
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WB
1/1000 - 1/2000. Predicted molecular weight: 56 kDa. |
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IP
Use a concentration of 3 µg/ml. |
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ICC/IF
1/20 - 1/200. |
ターゲット情報
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機能
Probable transcription factor. May have a role in myogenesis. -
関連疾患
Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator. -
配列類似性
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5081 Human
- Entrez Gene: 18509 Mouse
- Omim: 167410 Human
- SwissProt: P23759 Human
- SwissProt: P47239 Mouse
- Unigene: 113253 Human
- Unigene: 218760 Mouse
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別名
- FLJ37460 antibody
- HUP1 antibody
- OTTHUMP00000002534 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (ab187339)
Immunocytochemistry analysis of formalin-fixed HeLa cells permeabilized with 0.1% Triton X-100 staining PAX-7 in the nucleus with ab187339 in a 1/100 dilution (green). Secondary was DyLight-conjugated secondary antibody. Negative control (left), had no primary antibody. F-actin was stianed with fluorescent red phalloidin, and nuclei were stained with DAPI or Hoechst.
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Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (ab187339)Immunocytochemistry analysis of formalin-fixed MCF-7 cells (human breast adenocarcinoma cell line) permeabilized with 0.1% Triton X-100 staining PAX-7 in the nucleus with ab187339 in a 1/100 dilution (green). Secondary was DyLight-conjugated secondary antibody. Negative control (left), had no primary antibody. F-actin was stianed with fluorescent red phalloidin, and nuclei were stained with DAPI or Hoechst.
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Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (ab187339)Immunocytochemistry analysis of formalin-fixed C2C12 cells (mouse myoblast cell line) permeabilized with 0.1% Triton X-100 staining PAX-7 in the nucleus with ab187339 in a 1/100 dilution (green). Secondary was DyLight-conjugated secondary antibody. Negative control (left), had no primary antibody. F-actin was stianed with fluorescent red phalloidin, and nuclei were stained with DAPI or Hoechst.
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Western blot - Anti-PAX7 antibody (ab187339)All lanes : Anti-PAX7 antibody (ab187339) at 1/1000 dilution
Lane 1 : HeLa lysate transfected with empty vector control
Lane 2 : HeLa lysate overexpressing PAX7
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa -
Western blot - Anti-PAX7 antibody (ab187339)All lanes : Anti-PAX7 antibody (ab187339) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF7 whole cell lysate
Lane 3 : HSkMSC whole cell lysate
Lane 4 : SJCRH30 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (ab187339)Immunofluorescent analysis of PAX7 in MCF7 (left panel) and HeLa (right panel) cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were probed with a ab187339 at 1/100 dilution for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
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Immunoprecipitation - Anti-PAX7 antibody (ab187339)Immunoprecipitation of PAX7 was performed using HeLa whole cell lysate. Antigen-antibody complexes were formed by incubating 500µg of lysate with 3µg of ab187339 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted. Samples was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with ab187339 at 1/1000 dilution overnight rotating at 4°C.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab187339 は 19 報の論文で使用されています。
- McNamara SL et al. Anti-inflammatory therapy enables robot-actuated regeneration of aged muscle. Sci Robot 8:eadd9369 (2023). PubMed: 36947599
- Nam S et al. Active tissue adhesive activates mechanosensors and prevents muscle atrophy. Nat Mater 22:249-259 (2023). PubMed: 36357687
- Jin Z et al. Role of skeletal muscle satellite cells in the repair of osteoporotic fractures mediated by β-catenin. J Cachexia Sarcopenia Muscle 13:1403-1417 (2022). PubMed: 35178895
- Hao R et al. Differences in inferior oblique muscle satellite cell populations between primary and secondary inferior oblique muscle overaction. Ophthalmic Res N/A:N/A (2022). PubMed: 36302348
- Boesch J et al. Chemokine-like receptor 1 plays a critical role in modulating the regenerative and contractile properties of muscle tissue. Front Physiol 13:1044488 (2022). PubMed: 36467705