AB15164

Anti-PAX5 抗体

Name: Anti-PAX5 抗体 (ab15164) | Abcam
Brand: Abcam Limited
SKU: AB15164
Rating: 5 (2 reviews)

Anti-PAX5 antibody

(2 Reviews)

(7 Publications)

Rabbit Polyclonal PAX5 antibody. Suitable for IP, IHC-P and reacts with Human samples. Cited in 7 publications.

別名を表示する

Paired box protein Pax-5, B-cell-specific transcription factor, BSAP, PAX5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody (AB15164)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody (AB15164)

ab15164 staining PAX5 in human tonsil by Immunohistochemistry (FFPE-sections).

Lab

Immunoprecipitation - Anti-PAX5 antibody (AB15164)

PAX5 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5μg of Rabbit polyclonal to PAX5 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).

The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15164.

Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

Band : 42kDa; PAX5

All lanes:

Immunoprecipitation - Anti-PAX5 antibody (ab15164)

Predicted band size: 42 kDa

true

Exposure time: 20min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PAX5 also called B-cell-specific activator protein (BSAP) plays an important role in regulating gene expression during B-cell development. PAX5 is a transcription factor with an approximate mass of 50-55 kDa. It expresses mainly in B cells and in certain regions of the brain and testis. This protein binds DNA and controls the transcription of genes necessary for B-cell commitment and identity. Through processes like alternative splicing PAX5 generates multiple isoforms that contribute to various functional roles in its expressed locations.

Biological function summary

PAX5 regulates the differentiation and maintenance of B-lymphocytes. It controls various genes critical for B-cell lineage commitment while repressing those involved in other lineages like T-cells and myeloid cells. PAX5 is part of a larger transcriptional complex interacting with other transcription factors to perform its function. During B-cell maturation PAX5 activates a specific gene set that helps shape the B-cell receptor repertoire a process important for effective immune responses.

Pathways

PAX5 integrates into key pathways such as the B-cell receptor signaling and JAK-STAT pathways. These pathways regulate processes like cell proliferation differentiation and apoptosis. In the B-cell receptor signaling PAX5 works alongside proteins like CD19 and BTK facilitating the transmission of activation signals necessary for B-cell survival. Connections in the JAK-STAT pathway involve interactions that promote gene expression changes necessary for B-cell functional activity further modulating the immune system's readiness.

PAX5 is significantly associated with B-cell acute lymphoblastic leukemia (B-ALL) and non-Hodgkin lymphoma. In B-ALL mutations or deletions in PAX5 often lead to impaired B-cell development and uncontrolled cell proliferation. PAX5 mutations influence proteins like EBF1 and IL7R which are pivotal in B-cell progenitor activity and leukemogenesis. abnormalities in PAX5 expression or function can disrupt normal immune function leading to B-cell associated cancers and impairing immune surveillance highlighting its importance in maintaining immune system health.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed : 10811620, PubMed : 27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed : 10811620, PubMed : 27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed : 32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed : 32612238).. (Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription (PubMed : 31941781). Participates also in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity (PubMed : 23678172).

See full target information PAX5

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Science advances 8:eadd6403 PubMed36516256

2022

PAX5 epigenetically orchestrates CD58 transcription and modulates blinatumomab response in acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhen Li,Takaya Moriyama,Satoshi Yoshimura,Xujie Zhao,Zhenhua Li,Xu Yang,Elisabeth Paietta,Mark R Litzow,Marina Konopleva,Jiyang Yu,Hiroto Inaba,Raul C Ribeiro,Ching-Hon Pui,Jun J Yang

PloS one 16:e0257977 PubMed34570823

2021

Transcription factors Krüppel-like factor 4 and paired box 5 regulate the expression of the Grainyhead-like genes.

Applications

Unspecified application

Species

Unspecified reactive species

Grzegorz Kotarba,Agnieszka Taracha-Wisniewska,Michal Miller,Michal Dabrowski,Tomasz Wilanowski

Journal of toxicologic pathology 34:161-180 PubMed33976473

2021

Databases for technical aspects of immunohistochemistry: 2021 update.

Applications

Unspecified application

Species

Unspecified reactive species

Takayasu Moroki,Saori Matsuo,Hirofumi Hatakeyama,Seigo Hayashi,Izumi Matsumoto,Satoshi Suzuki,Takashi Kotera,Kazuyoshi Kumagai,Kiyokazu Ozaki

Nature cancer 1:329-344 PubMed32885175

2020

Integrative genomic analyses reveal mechanisms of glucocorticoid resistance in acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Robert J Autry,Steven W Paugh,Robert Carter,Lei Shi,Jingjing Liu,Daniel C Ferguson,Calvin E Lau,Erik J Bonten,Wenjian Yang,J Robert McCorkle,Jordan A Beard,John C Panetta,Jonathan D Diedrich,Kristine R Crews,Deqing Pei,Christopher J Coke,Sivaraman Natarajan,Alireza Khatamian,Seth E Karol,Elixabet Lopez-Lopez,Barthelemy Diouf,Colton Smith,Yoshihiro Gocho,Kohei Hagiwara,Kathryn G Roberts,Stanley Pounds,Steven M Kornblau,Wendy Stock,Elisabeth M Paietta,Mark R Litzow,Hiroto Inaba,Charles G Mullighan,Sima Jeha,Ching-Hon Pui,Cheng Cheng,Daniel Savic,Jiyang Yu,Charles Gawad,Mary V Relling,Jun J Yang,William E Evans

Journal of toxicologic pathology 30:79-107 PubMed28190929

2017

Databases for technical aspects of immunohistochemistry.

Applications

IHC

Species

Dog

Satoshi Furukawa,Mika Nagaike,Kiyokazu Ozaki

Nucleic acids research 45:5086-5099 PubMed28158851

2017

p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Perearnau,Serena Orlando,Abul B M M K Islam,Edurne Gallastegui,Jonatan Martínez,Albert Jordan,Anna Bigas,Rosa Aligué,Maria Jesús Pujol,Oriol Bachs

The Journal of biological chemistry 287:27396-406 PubMed22736761

2012

Contribution of microRNA-1275 to Claudin11 protein suppression via a polycomb-mediated silencing mechanism in human glioma stem-like cells.

Applications

Species

Human

Keisuke Katsushima,Keiko Shinjo,Atsushi Natsume,Fumiharu Ohka,Makiko Fujii,Hirotaka Osada,Yoshitaka Sekido,Yutaka Kondo

View all publications

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AB137872

Anti-LEF1 antibody [EPR2029Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (AB137872)

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