AB124257

Anti-Pat1b 抗体

Name: Anti-Pat1b 抗体 (ab124257) | Abcam
Brand: Abcam Limited
SKU: AB124257
Price: 74000 JPY
Availability: InStock

Anti-Pat1b antibody

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(2 Publications)

Rabbit Polyclonal Pat1b antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PATL1 aa 400 to C-terminus.

別名を表示する

OK/KNS-cl.5, PATL1, Protein PAT1 homolog 1, PAT1-like protein 1, Protein PAT1 homolog b, Pat1b, hPat1b

2 Images

Unknown

Immunoprecipitation - Anti-Pat1b antibody (AB124257)

ab124257 at 1 μg/ml staining Pat1b in HeLa whole cell lysate immunoprecipitated using ab124257 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Pat1b antibody (ab124257)

Predicted band size: 87 kDa

false

Unknown

Western blot - Anti-Pat1b antibody (AB124257)

All lanes:

Western blot - Anti-Pat1b antibody (ab124257) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 87 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human PATL1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q86TB9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Pat1b also known as Processing body component 4 (PATI-4) is a protein with a mass of approximately 73 kDa. It functions mechanically as an RNA-binding protein involved in mRNA decay. Pat1b enhances mRNA decay by promoting decapping and subsequently exonucleolytic degradation. It is expressed in various human tissues with prominent activity reported in the brain and testes. In molecular biology labs Pat1b is regularly studied due to its central role in mRNA turnover.

Biological function summary

The degradation of mRNA represents a critical step where Pat1b plays an essential role. As a component of the Pat1-Lsm complex Pat1b interacts with several proteins to regulate the stability and translation of mRNA. Pat1b influences the mRNA decapping process which is vital for controlled mRNA degradation thereby controlling gene expression post-transcriptionally. This process ensures the removal of aberrant or unnecessary mRNAs from the cellular environment enabling the cell to adapt to new conditions or signals rapidly.

Pathways

MRNA decay and processing pathways involve Pat1b as a central component. Within the mRNA surveillance pathway Pat1b cooperates with Dcp1-Dcp2 decapping enzymes to mediate mRNA decay. Pat1b is pivotal in the nonsense-mediated mRNA decay (NMD) pathway where it partners with Upf1 to target and degrade mRNAs containing premature stop codons. These pathways ensure cellular integrity and protein synthesis regulation.

Disruptions in Pat1b are linked to neurodegenerative diseases such as Amyotrophic lateral sclerosis (ALS) and conditions like intellectual disability. In ALS a failure in precise mRNA surveillance and decay pathways leads to toxic buildup of improperly processed mRNAs implicating Pat1b along with proteins such as TDP-43. In the context of intellectual disabilities altered mRNA metabolism involving Pat1b and its interaction with proteins like FMRP can affect neuronal function and development highlighting its significance for normal cognitive function.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs (PubMed : 17936923, PubMed : 20543818, PubMed : 20584987, PubMed : 20852261). Acts as a scaffold protein that connects deadenylation and decapping machinery (PubMed : 17936923, PubMed : 20543818, PubMed : 20584987, PubMed : 20852261). Required for cytoplasmic mRNA processing body (P-body) assembly (PubMed : 17936923, PubMed : 20543818, PubMed : 20584987, PubMed : 20852261).. (Microbial infection) In case of infection, required for translation and replication of hepatitis C virus (HCV).

See full target information PATL1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 73:314-324.e4 PubMed30527663

2018

The Pat1-Lsm Complex Stabilizes ATG mRNA during Nitrogen Starvation-Induced Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Damián Gatica,Guowu Hu,Xu Liu,Nannan Zhang,Peter R Williamson,Daniel J Klionsky

Nature chemical biology 13:174-180 PubMed27918561

2016

A human microprotein that interacts with the mRNA decapping complex.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia G D'Lima,Jiao Ma,Lauren Winkler,Qian Chu,Ken H Loh,Elizabeth O Corpuz,Bogdan A Budnik,Jens Lykke-Andersen,Alan Saghatelian,Sarah A Slavoff

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pat1b 抗体

Anti-Pat1b antibody

Immunoprecipitation - Anti-Pat1b antibody (AB124257)

Western blot - Anti-Pat1b antibody (AB124257)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise