Anti-PASK 抗体 (ab114988)
Key features and details
- Rabbit polyclonal to PASK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PASK antibody
PASK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PASK -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee
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免疫原
Synthetic peptide, corresponding to a region within amino acids 425-475 of Human PASK (NP_055963.2).
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ポジティブ･コントロール
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab114988 was affinity purified using an epitope specific to PASK immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab114988の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 143 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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組織特異性
Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 2 PAS (PER-ARNT-SIM) domains.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on Thr-1161 and Thr-1165. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 23178 Human
- Omim: 607505 Human
- SwissProt: Q96RG2 Human
- Unigene: 397891 Human
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別名
- DKFZP434O051 antibody
- DKFZp686P2031 antibody
- hPASK antibody
see all
画像
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Western blot - Anti-PASK antibody (ab114988)All lanes : Anti-PASK antibody (ab114988) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 143 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-PASK antibody (ab114988)Detection of PASK by Western Blot of Immunprecipitate.
ab114988 at 0.4µg/ml staining PASK in HeLa whole cell lysate immunoprecipitated using ab114988 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 mminutes.
All lanes : Anti-PASK antibody (ab114988) at 0.4 µg/ml
Lane 1 : HeLa cell lysate, IP with ab114988 at 6 µg per mg lysate
Lane 2 : HeLa cell lysate, IP with alternative PASK antibody
Lane 3 : HeLa cell lysate, IP with control IgG
Lysates/proteins at 200 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab114988 は論文での使用が確認できていません。