Anti-Parvalbumin 抗体 (ab32895)
Key features and details
- Goat polyclonal to Parvalbumin
- Suitable for: WB, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Parvalbumin antibody
Parvalbumin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Parvalbumin -
由来種
Goat -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Xenopus laevis, Macaque monkey
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免疫原
Synthetic peptide corresponding to Human Parvalbumin aa 99-110 (C terminal).
Sequence:GVDEFSTLVAES
Database link: P20472 -
ポジティブ･コントロール
- WB: Human cerebellum and rat brain tissue lysate. Flow Cyt: Kelly cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32895の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.01 - 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
Block using milk. Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3µg/ml. |
| Flow Cyt |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.01 - 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). Block using milk. Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3µg/ml. |
|
Flow Cyt
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. -
配列類似性
Belongs to the parvalbumin family.
Contains 2 EF-hand domains. - Information by UniProt
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参照データベース
- Entrez Gene: 5816 Human
- Entrez Gene: 19293 Mouse
- Entrez Gene: 25269 Rat
- Omim: 168890 Human
- SwissProt: P20472 Human
- SwissProt: P80050 Macaque monkey
- SwissProt: P32848 Mouse
- SwissProt: P02625 Rat
see all -
別名
- D22S749 antibody
- MGC116759 antibody
- Parvalbumin alpha antibody
see all
画像
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Western blot - Anti-Parvalbumin antibody (ab32895)Lanes 1-2 : Anti-Parvalbumin antibody (ab32895) at 0.01 µg/ml
Lanes 3-4 : Anti-Parvalbumin antibody (ab32895) at 0.3 µg/ml
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human cerebellum tissue lysate + peptide
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat brain tissue lysate + peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 12 kDaLysate in RIPA buffer. Detected by chemiluminescence.
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Flow Cytometry - Anti-Parvalbumin antibody (ab32895)Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (10)
ab32895 は 10 報の論文で使用されています。
- Costantini I et al. Large-scale, cell-resolution volumetric mapping allows layer-specific investigation of human brain cytoarchitecture. Biomed Opt Express 12:3684-3699 (2021). PubMed: 34221688
- Cooke JE et al. Contrast gain control occurs independently of both parvalbumin-positive interneuron activity and shunting inhibition in auditory cortex. J Neurophysiol 123:1536-1551 (2020). PubMed: 32186432
- Defourny J et al. Pejvakin-mediated pexophagy protects auditory hair cells against noise-induced damage. Proc Natl Acad Sci U S A 116:8010-8017 (2019). PubMed: 30936319
- Eshima H et al. Dysfunction of muscle contraction with impaired intracellular Ca2+ handling in skeletal muscle and the effect of exercise training in male db/db mice. J Appl Physiol (1985) N/A:N/A (2018). PubMed: 30433865
- Staton CD et al. Orexin 2 receptor stimulation enhances resilience, while orexin 2 inhibition promotes susceptibility, to social stress, anxiety and depression. Neuropharmacology 143:79-94 (2018). PubMed: 30240784
- Damborsky JC et al. Local cholinergic-GABAergic circuitry within the basal forebrain is modulated by galanin. Brain Struct Funct 222:1385-1400 (2017). PubMed: 27496091
- Eshima H et al. Long-term, but not short-term high-fat diet induces fiber composition changes and impaired contractile force in mouse fast-twitch skeletal muscle. Physiol Rep 5:N/A (2017). PubMed: 28408640
- Eshima H et al. Improved skeletal muscle Ca2+ regulation in vivo following contractions in mice overexpressing PGC-1a. Am J Physiol Regul Integr Comp Physiol 312:R1017-R1028 (2017). PubMed: 28438761
- Iaccarino HF et al. Gamma frequency entrainment attenuates amyloid load and modifies microglia. Nature 540:230-235 (2016). PubMed: 27929004
- Tomer R et al. Advanced CLARITY for rapid and high-resolution imaging of intact tissues. Nat Protoc 9:1682-97 (2014). PubMed: 24945384
