Anti-PARVA (phospho S8) 抗体 [EPR2174] (ab79409)
![Western blot - Anti-PARVA (phospho S8) antibody [EPR2174] (ab79409)](https://www.abcam.co.jp/ps/products/79/ab79409/Images/Parvin-alpha-Primary-antibodies-ab79409-1.jpg "Western blot - Anti-PARVA (phospho S8) antibody [EPR2174] (ab79409)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2174] to PARVA (phospho S8)
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PARVA (phospho S8) antibody [EPR2174]
PARVA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2174] to PARVA (phospho S8) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- A549 cell lysate. HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2174 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79409の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
|
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| WB |
1/2500 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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| 特記事項 |
|---|
|
ICC/IF
1/100 - 1/250. |
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WB
1/2500 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.
ターゲット情報
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機能
Probably plays a role in the regulation of cell adhesion and cytoskeleton organization. Plays a role in ciliogenesis. -
組織特異性
Widely expressed, with highest levels in heart, skeletal muscle, kidney and liver. -
配列類似性
Belongs to the parvin family.
Contains 2 CH (calponin-homology) domains. -
細胞内局在
Cell junction > focal adhesion. Cell membrane. Cytoplasm > cytoskeleton. Constituent of focal adhesions. - Information by UniProt
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参照データベース
- Entrez Gene: 55742 Human
- Entrez Gene: 57342 Mouse
- Entrez Gene: 57341 Rat
- Omim: 608120 Human
- SwissProt: Q9NVD7 Human
- SwissProt: Q9EPC1 Mouse
- SwissProt: Q9HB97 Rat
- Unigene: 432914 Human
see all -
別名
- Actopaxin antibody
- Alpha parvin antibody
- Alpha-parvin antibody
see all
画像
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Western blot - Anti-PARVA (phospho S8) antibody [EPR2174] (ab79409)All lanes : Anti-PARVA (phospho S8) antibody [EPR2174] (ab79409) at 1/5000 dilution
Lane 1 : A549 cell lysate
Lane 2 : A549 cell lysate treated with LP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PARVA (phospho S8) antibody [EPR2174] (ab79409)](https://www.abcam.co.jp/ps/products/79/ab79409/Images/Parvin-alpha-Primary-antibodies-ab79409-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PARVA (phospho S8) antibody [EPR2174] (ab79409)Immunofluorescent staining of HeLa cells using ab79409 at 1/100 dilution. -
![Anti-PARVA (phospho S8) antibody [EPR2174] (ab79409)](https://www.abcam.co.jp/ps/products/79/ab79409/Images/ab79409-3-benefits-of-recombinant-antibodies.png)
Anti-PARVA (phospho S8) antibody [EPR2174] (ab79409)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab79409 は論文での使用が確認できていません。