Anti-PARP14 抗体 [EPR26299-91] (ab302710)
![Western blot - Anti-PARP14 antibody [EPR26299-91] (AB302710)](https://www.abcam.co.jp/ps/products/302/ab302710/Images/AB302710-489540-anti-parp14-antibody-epr2629991-western-blot-bxpc3.jpg "Western blot - Anti-PARP14 antibody [EPR26299-91] (AB302710)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26299-91] to PARP14
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PARP14 antibody [EPR26299-91]
PARP14 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR26299-91] to PARP14 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Whole cell lysates: BxPC-3 (human pancreas adenocarcinoma epithelial cell), SW480 (human colorectal adenocarcinoma epithelial cell); Human spleen and tonsil tissue lysates.
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特記事項
ab302710 does not react in: WB, ICC, and intracellular flow cytometry with mouse and rat species; IHC-P with human, mouse, and rat species, IP with human samples.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR26299-91 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302710の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 203 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 203 kDa). |
ターゲット情報
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機能
Enhances STAT6-dependent transcription (By similarity). Has ADP-ribosyltransferase activity. -
配列類似性
Contains 3 Macro domains.
Contains 1 PARP catalytic domain.
Contains 1 WWE domain. -
細胞内局在
Nucleus. Cytoplasm. In steady state cells the protein is mostly nuclear. A minor proportion is detected in the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 54625 Human
- Omim: 610028 Human
- SwissProt: Q460N5 Human
- Unigene: 518203 Human
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別名
- ADP-ribosyltransferase diphtheria toxin-like 8 antibody
- ARTD8 antibody
- B aggressive lymphoma protein 2 antibody
see all
画像
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Western blot - Anti-PARP14 antibody [EPR26299-91] (AB302710)All lanes : Anti-PARP14 antibody [EPR26299-91] (ab302710) at 1/1000 dilution
Lane 1 : BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 203 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Negative control: Raji (PMID: 23045269).
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![Western blot - Anti-PARP14 antibody [EPR26299-91] (AB302710)](https://www.abcam.co.jp/ps/products/302/ab302710/Images/AB302710-489541-anti-parp14-antibody-epr2629991-western-blot-human-spleen.jpg)
Western blot - Anti-PARP14 antibody [EPR26299-91] (AB302710)All lanes : Anti-PARP14 antibody [EPR26299-91] (ab302710) at 1/1000 dilution
Lane 1 : Human spleen tissue lysate
Lane 2 : Human tonsil tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 203 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1: 180 seconds, Lane 2: 70 seconds
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![Anti-PARP14 antibody [EPR26299-91] (AB302710)](https://www.abcam.co.jp/ps/products/302/ab302710/Images/AB302710-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-PARP14 antibody [EPR26299-91] (AB302710)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab302710 は論文での使用が確認できていません。