Anti-PARP1 抗体 [Y17] (ab32378)
![Western blot - Anti-PARP1 antibody [Y17] (ab32378)](https://www.abcam.co.jp/ps/products/32/ab32378/Images/ab32378-535049-anti-parp1-antibody-y17-western-blot-human-jurkat.jpg "Western blot - Anti-PARP1 antibody [Y17] (ab32378)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y17] to PARP1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PARP1 antibody [Y17]
PARP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y17] to PARP1 -
由来種
Rabbit -
特異性
This antibody is specific to PARP1. It should recognize the intact form (116kDa) and the p25 cleaved form of PARP1. -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Chinese hamster
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Jurkat cell lysate.
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特記事項
This product has switched from a hybridoma to recombinant production method on 23rd February 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y17 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32378の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 113 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 113 kDa). |
ターゲット情報
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機能
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. -
配列類似性
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
翻訳後修飾
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: P09874 Human
- Unigene: 177766 Human
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別名
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase antibody
- ADP ribosyltransferase diphtheria toxin like 1 antibody
see all
画像
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Western blot - Anti-PARP1 antibody [Y17] (ab32378)Anti-PARP1 antibody [Y17] (ab32378) at 1/1000 dilution + Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 113 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Exposure time: 7 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab32378 は 2 報の論文で使用されています。
- Chi YH et al. Discovery and Synthesis of a Pyrimidine-Based Aurora Kinase Inhibitor to Reduce Levels of MYC Oncoproteins. J Med Chem 64:7312-7330 (2021). PubMed: 34009981
- Zhao J et al. 2,3,5,4'-tetrahydroxystilbene-2-O-ß-D-glucoside protects human umbilical vein endothelial cells against lysophosphatidylcholine-induced apoptosis by upregulating superoxide dismutase and glutathione peroxidase. IUBMB Life 66:711-22 (2014). PubMed: 25382724