Name: Anti-PARP1 antibody [EPR18461]
Brand: RabMAb
SKU: ab191217
Rating: 4 (6 reviews)

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Western blot - Anti-PARP1 antibody [EPR18461] (ab191217)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18461] to PARP1
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Carrier Free

製品名

Anti-PARP1 antibody [EPR18461]
PARP1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR18461] to PARP1
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-P, ICC/IFmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: HeLa and NIH/3T3 whole cell lysates; Human fetal heart and fetal kidney lysates; Mouse heart lysate; Rat brain and heart lysates. IHC-P: Human, mouse and rat testis tissues. ICC/IF: HeLa and NIH/3T3 cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR18461
アイソタイプ

IgG
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab191217の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(3)	1/1000. Detects a band of approximately 113, 89, 55 kDa (predicted molecular weight: 113 kDa).
IHC-P	(1)	1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF	(1)	1/500.

特記事項
WB 1/1000. Detects a band of approximately 113, 89, 55 kDa (predicted molecular weight: 113 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.

機能

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
配列類似性

Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
翻訳後修飾

Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
細胞内局在

Nucleus.
Target information above from: UniProt accession P09874 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 142 Human
- Entrez Gene: 11545 Mouse
- Entrez Gene: 25591 Rat
- Omim: 173870 Human
- SwissProt: P09874 Human
- SwissProt: P11103 Mouse
- SwissProt: P27008 Rat
- Unigene: 177766 Human
- Unigene: 277779 Mouse
- Unigene: 11327 Rat
see all
別名
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase antibody
- ADP ribosyltransferase diphtheria toxin like 1 antibody
- ADP ribosyltransferase NAD(+) antibody
- ADPRT 1 antibody
- ADPRT antibody
- ADPRT1 antibody
- ARTD1 antibody
- msPARP antibody
- NAD(+) ADP ribosyltransferase 1 antibody
- NAD(+) ADP-ribosyltransferase 1 antibody
- pADPRT 1 antibody
- pADPRT-1 antibody
- pADPRT1 antibody
- PARP 1 antibody
- PARP antibody
- PARP-1 antibody
- PARP1 antibody
- PARP1_HUMAN antibody
- Poly (ADP ribose) polymerase 1 antibody
- poly (ADP ribose) polymerase family, member 1 antibody
- Poly (ADP-ribose) polymerase 1 antibody
- Poly [ADP-ribose] polymerase 1 antibody
- Poly(ADP ribose) polymerase antibody
- poly(ADP ribose) synthetase antibody
- poly(ADP ribosyl)transferase antibody
- Poly(ADP-ribosyl)transferase antibody
- Poly[ADP ribose] synthetase 1 antibody
- Poly[ADP-ribose] synthase 1 antibody
- PPOL antibody
- sPARP 1 antibody
- sPARP1 antibody
see all

Western blot - Anti-PARP1 antibody [EPR18461] (ab191217)

All lanes : Anti-PARP1 antibody [EPR18461] (ab191217) at 1/1000 dilution

Lane 1 : Wild-type A549 control staurosporine (0 uM, 72 h) cell lysate at 20 µg
Lane 2 : Wild-type A549 treated staurosporine (3 uM, 24 h) cell lysate at 20 µg
Lane 3 : PARP1 knockout A549 control staurosporine (0 uM, 72 h) cell lysate at 20 µg
Lane 4 : PARP1 knockout A549 treated staurosporine (3 uM, 24 h) cell lysate at 20 µg
Lane 5 : Empty cell lysate at 20 µg
Lane 6 : HAP1 Treated Staurosporine (2uM, 4h) cell lysate at 20 µg
Lane 7 : HAP1 Vehicle Control Staurosporine (0uM, 4h) cell lysate at 10 µg

Performed under reducing conditions.

Predicted band size: 113 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?

Western blot: Anti-PARP1 antibody [EPR18461] (ab191217) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab191217 was shown to bind specifically to PARP1. A band was observed at 125 kDa in wild-type A549 cell lysates with no signal observed at this size in PARP1 knockout cell line ab276094 (knockout cell lysate Abcam Pools). To generate this image, wild-type and PARP1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
Western blot - Anti-PARP1 antibody [EPR18461] (ab191217)

All lanes : Anti-PARP1 antibody [EPR18461] (ab191217) at 1/1000 dilution

Lane 1 : Rat brain lysates prepared in RIPA lysis method
Lane 2 : Rat brain lysates prepared in 1%SDS Hot lysis method

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 113 kDa

The lysates were prepared in 1%SDS Hot lysis method.

Blocking/diluting buffer & concentration: 5% NFDM/TBST

Observed MW: 112 kDa
Western blot - Anti-PARP1 antibody [EPR18461] (ab191217)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: PARP1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab191217 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab191217 was shown to specifically react with PARP1 when PARP1 knockout samples were used. Wild-type and PARP1 knockout samples were subjected to SDS-PAGE. ab191217 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody [EPR18461] (ab191217)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PARP1 with ab191217 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on epithelial cells and stromal cells of Human testis is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-PARP1 antibody [EPR18461] (ab191217)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PARP1 with ab191217 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on HeLa cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:-
-ve control 1: ab191217 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-PARP1 antibody [EPR18461] (ab191217)

All lanes : Anti-PARP1 antibody [EPR18461] (ab191217) at 1/10000 dilution

Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 1uM staurosporine for 4 hours whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/50000 dilution

Predicted band size: 113 kDa
Observed band size: 113,89 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID: 1536009).

The lysates were prepared in 1%SDS Hot lysis method
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody [EPR18461] (ab191217)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling PARP1 with ab191217 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on epithelial cells and stromal cells of rat testis is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-PARP1 antibody [EPR18461] (ab191217)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cells) cells labeling PARP1 with ab191217 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (red).

The negative controls are as follows:-
-ve control 1: ab191217 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-PARP1 antibody [EPR18461] (ab191217)

All lanes : Anti-PARP1 antibody [EPR18461] (ab191217) at 1/10000 dilution

Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cells) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cells) treated with 1uM staurosporine for 4 hours whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 113 kDa
Observed band size: 113,55,89 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID: 1536009).

The lysates were prepared in 1%SDS Hot lysis method
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody [EPR18461] (ab191217)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling PARP1 with ab191217 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on epithelial cells and stromal cells of mouse testis is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-PARP1 antibody [EPR18461] (ab191217)

All lanes : Anti-PARP1 antibody [EPR18461] (ab191217) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 113 kDa
Observed band size: 113 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The lysates were prepared in 1%SDS Hot lysis method
Western blot - Anti-PARP1 antibody [EPR18461] (ab191217)

Anti-PARP1 antibody [EPR18461] (ab191217) at 1/1000 dilution + Mouse heart lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/50000 dilution

Predicted band size: 113 kDa
Observed band size: 113 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.

The lysates were prepared in 1%SDS Hot lysis method
Anti-PARP1 antibody [EPR18461] (ab191217)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

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Datasheet download

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ab191217 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab191217 は 85 報の論文で使用されています。

Liu L et al. DERL2 (derlin 2) stabilizes BAG6 (BAG cochaperone 6) in chemotherapy resistance of cholangiocarcinoma. J Physiol Biochem 80:81-97 (2024). PubMed: 37815698
Liu Y et al. Regulation of NAD+/NADH Redox Involves the Protective Effects of Ginsenoside Rb1 against Oxygen-Glucose Deprivation/Reoxygenation-Induced Astrocyte Lesions. Int J Mol Sci 24:N/A (2023). PubMed: 38003249
Li M et al. circCIMT Silencing Promotes Cadmium-Induced Malignant Transformation of Lung Epithelial Cells Through the DNA Base Excision Repair Pathway. Adv Sci (Weinh) 10:e2206896 (2023). PubMed: 36814305
Wang X et al. PET imaging of PARP expression using 68Ga-labelled inhibitors. Eur J Nucl Med Mol Imaging 50:2606-2620 (2023). PubMed: 37145164
Wu J et al. A cell transmembrane peptide chimeric M(27-39)-HTPP targeted therapy for hepatocellular carcinoma. iScience 26:106766 (2023). PubMed: 37234089

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-6 of 6 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Lung cancer)

Gel Running Conditions

Reduced Denaturing (4-12)

Loading amount

40 µg

Specification

Lung cancer

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Marie Klimontova

Verified customer

投稿 Nov 04 2022

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Dog Tissue sections (Testis)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH=9

Permeabilization

Specification

Testis

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 30% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Sophie Favril

Verified customer

投稿 Mar 23 2021

Application

ELISA

Sample

Human Purified protein (Recombinant)

Specification

Recombinant

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Type

Direct

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 04 2021

Application

Western blot

Sample

Dog Cell lysate - whole cell (melanoma cell line)

Gel Running Conditions

Reduced Denaturing

Loading amount

10 µg

Specification

melanoma cell line

Blocking step

(agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. 令中野

Verified customer

投稿 Jun 17 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (HAP1 cell)

Gel Running Conditions

Reduced Denaturing (8)

Loading amount

100000 cells

Specification

HAP1 cell

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 22 2018

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (eHAP1)

Permeabilization

Yes - 0.1% TritonX-100

Specification

eHAP1

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 13 2018

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (85)

レビューと Q&A

Western blot abreview for Anti-PARP1 antibody [EPR18461]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PARP1 antibody [EPR18461]

ELISA abreview for Anti-PARP1 antibody [EPR18461]

Western blot abreview for Anti-PARP1 antibody [EPR18461]

Western blot abreview for Anti-PARP1 antibody [EPR18461]

Immunocytochemistry/ Immunofluorescence abreview for Anti-PARP1 antibody [EPR18461]