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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PARP1 抗体 [E78] (ab32071)

Reviews (2)Q&A (2)References (28)
Western blot - Anti-PARP1 antibody [E78] (ab32071)
  • Western blot - Anti-PARP1 antibody [E78] (ab32071)
  • Anti-PARP1 antibody [E78] (ab32071)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E78] to PARP1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PARP1 antibody [E78]
    PARP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [E78] to PARP1

  • 由来種

    Rabbit

  • 特異性

    ab32071 should recognise both pro-form and p85 cleaved-form of PARP1.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Jurkat whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32071の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/1000. Detects a band of approximately 85, 116 kDa (predicted molecular weight: 113 kDa).
特記事項
WB
1/1000. Detects a band of approximately 85, 116 kDa (predicted molecular weight: 113 kDa).

ターゲット情報

  • 機能

    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.

  • 配列類似性

    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.

  • 翻訳後修飾

    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
      • ADP ribosyltransferase antibody
      • ADP ribosyltransferase diphtheria toxin like 1 antibody
      • ADP ribosyltransferase NAD(+) antibody
      • ADPRT 1 antibody
      • ADPRT antibody
      • ADPRT1 antibody
      • ARTD1 antibody
      • msPARP antibody
      • NAD(+) ADP ribosyltransferase 1 antibody
      • NAD(+) ADP-ribosyltransferase 1 antibody
      • pADPRT 1 antibody
      • pADPRT-1 antibody
      • pADPRT1 antibody
      • PARP 1 antibody
      • PARP antibody
      • PARP-1 antibody
      • PARP1 antibody
      • PARP1_HUMAN antibody
      • Poly (ADP ribose) polymerase 1 antibody
      • poly (ADP ribose) polymerase family, member 1 antibody
      • Poly (ADP-ribose) polymerase 1 antibody
      • Poly [ADP-ribose] polymerase 1 antibody
      • Poly(ADP ribose) polymerase antibody
      • poly(ADP ribose) synthetase antibody
      • poly(ADP ribosyl)transferase antibody
      • Poly(ADP-ribosyl)transferase antibody
      • Poly[ADP ribose] synthetase 1 antibody
      • Poly[ADP-ribose] synthase 1 antibody
      • PPOL antibody
      • sPARP 1 antibody
      • sPARP1 antibody
      see all

    画像

    • Western blot - Anti-PARP1 antibody [E78] (ab32071)
      Western blot - Anti-PARP1 antibody [E78] (ab32071)
      All lanes : Anti-PARP1 antibody [E78] (ab32071) at 1/1000 dilution (Purified)

      Lane 1 : Untreated Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
      Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) treated with 1µM staurosporine for 4 hours whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 113 kDa



      pro-form: 116kDa; p85 caspases cleaved form: 85kDa; proteolysis cleaved fragments: 71kDa, 55kDa and 42kDa

    • Western blot - Anti-PARP1 antibody [E78] (ab32071)
      Western blot - Anti-PARP1 antibody [E78] (ab32071)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: PARP1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: MCF7 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green ab32071 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32071 was shown to specifically react with PARP1 when PARP1 knockout samples were used. Wild-type and PARP1 knockout samples were subjected to SDS-PAGE. ab32071 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10 000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-PARP1 antibody [E78] (ab32071)
      Anti-PARP1 antibody [E78] (ab32071)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (28)

    ab32071 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab32071 は 28 報の論文で使用されています。

    • Shin SH  et al. ITC-6102RO, a novel B7-H3 antibody-drug conjugate, exhibits potent therapeutic effects against B7-H3 expressing solid tumors. Cancer Cell Int 23:172 (2023). PubMed: 37596639
    • Hsu RJ  et al. Z-Ligustilide Induces c-Myc-Dependent Apoptosis via Activation of ER-Stress Signaling in Hypoxic Oral Cancer Cells. Front Oncol 12:824043 (2022). PubMed: 35494068
    • Amrenova A  et al. Cell competition between anaplastic thyroid cancer and normal thyroid follicular cells exerts reciprocal stress response defining tumor suppressive effects of normal epithelial tissue. PLoS One 16:e0249059 (2021). PubMed: 33793628
    • Kim IY  et al. Titanium Dioxide Induces Apoptosis under UVA Irradiation via the Generation of Lysosomal Membrane Permeabilization-Dependent Reactive Oxygen Species in HaCat Cells. Nanomaterials (Basel) 11:N/A (2021). PubMed: 34443774
    • Pisano M  et al. Anticancer Activity of Two Novel Hydroxylated Biphenyl Compounds toward Malignant Melanoma Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34073232
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-PARP1 antibody [E78]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (mouse neural stem cells)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    mouse neural stem cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Robert Kupp

    Verified customer

    投稿 Oct 19 2020

    Western blot abreview for Anti-PARP1 antibody [E78]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1 cell line)
    Gel Running Conditions
    Non-reduced Denaturing (4-12% Bis-Tris gel)
    Loading amount
    50 µg
    Specification
    HAP1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 10 2016

    Question

    Dear Sir/Madam The customer would like to PARP antibody that result same with the publication (page 1387, attach file) which have PARP protein and cleave form. So, please kindly recommend abcam antibody.

    Read More
    Answer

    Thank you for contacting us. We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? This can be done using ab75757 (Chicken polyclonal) or ab37722 (Rabbit polyclonal). Although ab37722 has not been tested with mouse samples due but to the similarity in immunogen sequence (100%) it is predicted to react. If however you are interested in human samples this can be achieved with ab32071 (Rabbit monoclonal [E78]) which was raised against a synthetic peptide of the C-teminal residues of human PARP. Please note this antibody does not react with mouse samples. We also have several antibodies that specifically recognise the cleavage product of PARP such as ab4830 (reacts with human C-terminal PARP) and ab32064 (reacts with mouse and human PARP, N-terminal). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    We are having difficulty with the antibody ab32071-100 PARP (E78), lot No.191268. My colleague has tried the recommended 1:1000 dilution and 1:500 in a Western Blot with Hela cell extract but saw no signal. An actin antibody confirmed the extract was good. Have you had any other difficulties with this batch? Normally we are very happy with Abcam antibodies.

    Read More
    Answer

    I'm sorry to hear you are experiencing problems with ab32071. You are the only person so far who has had problems with this product. There may be a number of reasons why the antibody is not working at the moment and I would like to offer to look at your protocol in details to see if the problem could be solved with a few modifications. I enclose below a link to a form to help you put your protocol information easily together, https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=32071&mode=questionaire I would also recommend to run a positive control of Jurkat cell lysate to make sure that the problem is not due to low levels of the proteins in HeLa cells. Please be assured that if the problem is due to the quality of the antibody we would be happy to offer you a refund or replacement vial if the antibody was purchased in the last 90 days.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com