Anti-PARP1 抗体 (ab227244)
Key features and details
- Rabbit polyclonal to PARP1
- Suitable for: ChIP, IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PARP1 antibody
PARP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PARP1 -
由来種
Rabbit -
アプリケーション
適用あり: ChIP, IHC-P, WB, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human PARP1 (internal sequence). The exact sequence is proprietary.
Database link: P09874 -
ポジティブ･コントロール
- WB: HEK-293T, NIH/3T3 and PC-12 whole cell extracts; HCT 116, HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900). ICC/IF: HeLa cells. ChIP: HeLa and Raji chromatin extracts. IP: HCT 116 whole cell extract. IHC-P: HeLa xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227244の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ChIP |
Use at an assay dependent concentration.
|
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| IHC-P |
1/100 - 1/1000.
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| WB | (4) |
1/500 - 1/10000. Predicted molecular weight: 113 kDa.
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| ICC/IF | (1) |
1/100 - 1/1000.
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| IP | (1) |
1/100 - 1/500.
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| 特記事項 |
|---|
|
ChIP
Use at an assay dependent concentration. |
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IHC-P
1/100 - 1/1000. |
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WB
1/500 - 1/10000. Predicted molecular weight: 113 kDa. |
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ICC/IF
1/100 - 1/1000. |
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IP
1/100 - 1/500. |
ターゲット情報
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機能
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. -
配列類似性
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
翻訳後修飾
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 142 Human
- Entrez Gene: 11545 Mouse
- Entrez Gene: 25591 Rat
- Omim: 173870 Human
- SwissProt: P09874 Human
- SwissProt: P11103 Mouse
- SwissProt: P27008 Rat
- Unigene: 177766 Human
see all -
別名
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase antibody
- ADP ribosyltransferase diphtheria toxin like 1 antibody
see all
画像
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Western blot - Anti-PARP1 antibody (ab227244)All lanes : Anti-PARP1 antibody (ab227244) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : PARP1 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 113 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-PARP1 antibody (ab227244)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PARP1 (green) using ab227244 at 1/500 dilution in ICC/IF.
Red: Phalloidin, a cytoskeleton marker, at 1/200 dilution.
Blue: Hoechst 33342 staining.
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ChIP - Anti-PARP1 antibody (ab227244)ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or ab227244. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP1 antibody (ab227244)Paraffin-embedded HeLa xenograft tissue stained for PARP1 using ab227244 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based, pH 8.0, buffer, 15minutes.
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Immunoprecipitation - Anti-PARP1 antibody (ab227244)PARP1 was immunoprecipitated from HCT 116 (human colorectal carcinoma cell line) whole cell extract with 4 µg ab227244. Western blot was performed from the immunoprecipitate using ab227244 at 1/500 dilution. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HCT 116 whole cell extract 30 μg.
Lane 2: Control IP in HCT 116 whole cell extract with 4 μg of preimmune Rabbit IgG.
Lane 3: ab227244 IP in HCT 116 whole cell extract.
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Western blot - Anti-PARP1 antibody (ab227244)All lanes : Anti-PARP1 antibody (ab227244) at 1/1000 dilution
Lane 1 : 293T whole cell extracts
Lane 2 : A431 whole cell extracts
Lane 3 : HeLa whole cell extracts
Lane 4 : HepG2 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 113 kDa5% gel.
Running conditions: 80V, 15min; 140V, 40 minutes.
Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.
Primary antibody incubation: 4? , overnight.
Washing condition: 5 ml TBST, 4 x 5 minutes.
Exposure: chemiluminescent substrate for the detection of HRP-conjugated antibody
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Western blot - Anti-PARP1 antibody (ab227244)All lanes : Anti-PARP1 antibody (ab227244) at 1/1000 dilution
All lanes : Neuro2A whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 113 kDa5% gel.
Running conditions: 80V, 15min; 140V, 40 minutes.
Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.
Primary antibody incubation: 4? , overnight.
Washing condition: 5 ml TBST, 4 x 5 minutes.
Exposure: chemiluminescent substrate for the detection of HRP-conjugated antibody
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Western blot - Anti-PARP1 antibody (ab227244)All lanes : Anti-PARP1 antibody (ab227244) at 1/5000 dilution
Lane 1 : HCT116 whole cell lysate (untreated)
Lane 2 : HCT116 whole cell lysate (30 µM cisplatin treatment for 24 hours)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 113 kDa7.5% SDS-PAGE gel.
The top band is full-length and the bottom band on lane 2 is cleaved PARP1.
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ChIP - Anti-PARP1 antibody (ab227244)Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or ab227244. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
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Western blot - Anti-PARP1 antibody (ab227244)All lanes : Anti-PARP1 antibody (ab227244) at 1/500 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 113 kDa7.5% SDS-PAGE gel.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (45)
ab227244 は 45 報の論文で使用されています。
- Brabson JP et al. Oxidized mC modulates synthetic lethality to PARP inhibitors for the treatment of leukemia. Cell Rep 42:112027 (2023). PubMed: 36848231
- Huang M et al. PARP1 negatively regulates transcription of BLM through its interaction with HSP90AB1 in prostate cancer. J Transl Med 21:445 (2023). PubMed: 37415147
- Zhou Z & Zhang H CHAC1 exacerbates LPS-induced ferroptosis and apoptosis in HK-2 cells by promoting oxidative stress. Allergol Immunopathol (Madr) 51:99-110 (2023). PubMed: 36916093
- Schaich MA et al. Single-molecule analysis of DNA-binding proteins from nuclear extracts (SMADNE). Nucleic Acids Res 51:e39 (2023). PubMed: 36861323
- Hong L et al. Tumor-associated macrophages promote cisplatin resistance in ovarian cancer cells by enhancing WTAP-mediated N6-methyladenosine RNA methylation via the CXCL16/CXCR6 axis. Cancer Chemother Pharmacol 92:71-81 (2023). PubMed: 37272931