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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PARK7/DJ1 抗体 [EP2816Y] (ab76241)

Reviews (1) Submit a question References (9)
Western blot - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)
  • Flow Cytometry (Intracellular) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2816Y] to PARK7/DJ1
  • Suitable for: WB, IHC-P, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PARK7/DJ1 antibody [EP2816Y]
    PARK7/DJ1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP2816Y] to PARK7/DJ1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, Flow Cyt (Intra)more details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Jurkat cells, Mouse and RAt brain cell lysates; Flow Cyt (intra): Jurkat cells. IHC-P: Human, Mouse and rat cerebrum tissue

  • 特記事項

    Production method for this product has been changed from hybridoma to recombinant on 15th August 2024

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76241の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/2000. Detects a band of approximately 24 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
WB
1/2000. Detects a band of approximately 24 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone.

  • 組織特異性

    Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain. Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.

  • 関連疾患

    Defects in PARK7 are the cause of Parkinson disease type 7 (PARK7) [MIM:606324]. A neurodegenerative disorder characterized by resting tremor, postural tremor, bradykinesia, muscular rigidity, anxiety and psychotic episodes. PARK7 has onset before 40 years, slow progression and initial good response to levodopa. Some patients may show traits reminiscent of amyotrophic lateral sclerosis-parkinsonism/dementia complex (Guam disease).

  • 配列類似性

    Belongs to the peptidase C56 family.

  • 翻訳後修飾

    Sumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.
    Cys-106 is easily oxidized to sulfinic acid.
    Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.

  • 細胞内局在

    Cytoplasm. Nucleus. Mitochondrion. Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CAP1 antibody
    • DJ-1 antibody
    • DJ1 antibody
    • DJ1 protein antibody
    • Epididymis secretory sperm binding protein Li 67p antibody
    • FLJ27376 antibody
    • FLJ34360 antibody
    • FLJ92274 antibody
    • HEL S 67p antibody
    • Oncogene DJ1 antibody
    • OTTHUMP00000001348 antibody
    • OTTHUMP00000001349 antibody
    • OTTHUMP00000001350 antibody
    • OTTHUMP00000001351 antibody
    • PARK7 antibody
    • PARK7_HUMAN antibody
    • Parkinson disease (autosomal recessive, early onset) 7 antibody
    • Parkinson disease protein 7 antibody
    • Parkinson protein 7 antibody
    • Protein DJ-1 antibody
    • SP22 antibody
    see all

画像

  • Western blot - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)
    Western blot - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)
    All lanes : Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241) at 1/2000 dilution

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1, 10 seconds; Lane 2, 5 seconds; Lane 3, 3 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling PARK7/DJ1 with ab76241 at 1/500 (0.494 ug/ml) dilution.

    Positive staining on rat cerebrum tissue. The section was incubated with ab76241 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PARK7/DJ1 with ab76241 at 1/500 (0.494 ug/ml) dilution.

    Positive staining on mouse cerebrum tissue. The section was incubated with ab76241 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PARK7/DJ1 with ab76241 at 1/500 (0.494 ug/ml) dilution.

    Positive staining on human cerebrum tissue. The section was incubated with ab76241 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Flow Cytometry (Intracellular) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)
    Flow Cytometry (Intracellular) - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labelling PARK7/DJ1 with ab76241 at 1/20 dilution (7.5 ug/mL) (Red) compared with a isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

参考文献 (9)

ab76241 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76241 は 9 報の論文で使用されています。

  • Xia Q  et al. Peroxiredoxin 2 is required for the redox mediated adaptation to exercise. Redox Biol 60:102631 (2023). PubMed: 36791646
  • Goljanek-Whysall K  et al. miR-181a regulates p62/SQSTM1, parkin, and protein DJ-1 promoting mitochondrial dynamics in skeletal muscle aging. Aging Cell 19:e13140 (2020). PubMed: 32291905
  • Tsai CY  et al. Redox-active DJ-1 sustains brainstem cardiovascular regulation via maintenance of mitochondrial function during mevinphos intoxication. Neurochem Int 139:104791 (2020). PubMed: 32650030
  • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
  • Di Nottia M  et al. DJ-1 modulates mitochondrial response to oxidative stress: clues from a novel diagnosis of PARK7. Clin Genet N/A:N/A (2016). PubMed: 27460976
  • Prahlad J  et al. Use of cysteine-reactive cross-linkers to probe conformational flexibility of human DJ-1 demonstrates that Glu18 mutations are dimers. J Neurochem 130:839-53 (2014). PubMed: 24832775
  • Poschmann G  et al. High-fat diet induced isoform changes of the Parkinson's disease protein DJ-1. J Proteome Res 13:2339-51 (2014). PubMed: 24646099
  • Besong Agbo D  et al. Development of a capillary isoelectric focusing immunoassay to measure DJ-1 isoforms in biological samples. Anal Biochem 443:197-204 (2013). PubMed: 24055619
  • O'Connell K & Ohlendieck K Proteomic DIGE analysis of the mitochondria-enriched fraction from aged rat skeletal muscle. Proteomics 9:5509-24 (2009). PubMed: 19834913

レビューと Q&A

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Western blot abreview for Anti-PARK7/DJ1 antibody [EP2816Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (12,5%)
Loading amount
50 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Polina Vishnyakova

Verified customer

投稿 Mar 29 2019

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