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Anti-PARG 抗体 [OTI1B5] (ab236403)

Submit a review Submit a question References (1)
Western blot - Anti-PARG antibody [OTI1B5] (ab236403)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARG antibody [OTI1B5] (ab236403)

Key features and details

  • Mouse monoclonal [OTI1B5] to PARG
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-PARG antibody [OTI1B5]
    PARG 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [OTI1B5] to PARG

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment corresponding to Human PARG aa 1-400. Produced in E.coli (NP_003622).
    Database link: Q86W56

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: pCMV6-ENTRY PARG transfected HEK-293T cell lysate. IHC-P: Human tonsil tissue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    Purified from cell culture supernatant.

  • ポリ/モノ

    モノクローナル

  • クローン名

    OTI1B5

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab236403の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000. Predicted molecular weight: 111 kDa.
IHC-P
1/150.

Perform heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 °C for 3 minutes.
特記事項
WB
1/2000. Predicted molecular weight: 111 kDa.
IHC-P
1/150.

Perform heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 °C for 3 minutes.

ターゲット情報

  • 機能

    Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells.

  • 組織特異性

    Ubiquitously expressed.

  • 配列類似性

    Belongs to the poly(ADP-ribose) glycohydrolase family.

  • 細胞内局在

    Cytoplasm and Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ADP riboseglycohydrolase antibody
      • Mitochondrial poly(ADP ribose) glycohydrolase antibody
      • Parg antibody
      • PARG_HUMAN antibody
      • PARG99 antibody
      • Poly antibody
      • poly (ADP ribose) glycohydrolase antibody
      • poly(ADP-ribose) glycohydrolase 60 kDa isoform antibody
      • Poly(ADP-ribose) glycohydrolase antibody
      see all

    画像

    • Western blot - Anti-PARG antibody [OTI1B5] (ab236403)
      Western blot - Anti-PARG antibody [OTI1B5] (ab236403)
      All lanes : Anti-PARG antibody [OTI1B5] (ab236403) at 1/2000 dilution

      Lane 1 : pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
      Lane 2 : pCMV6-ENTRY PARG transfected HEK-293T cell lysate

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 111 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARG antibody [OTI1B5] (ab236403)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARG antibody [OTI1B5] (ab236403)

      Paraffin-embedded human tonsil tissue stained for PARG using ab236403 at 1/150 dilution in immunohistochemical analysis.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab236403 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab236403 は 1 報の論文で使用されています。

    • Peng B  et al. PARP1 and CHK1 coordinate PLK1 enzymatic activity during the DNA damage response to promote homologous recombination-mediated repair. Nucleic Acids Res 49:7554-7570 (2021). PubMed: 34197606

    レビューと Q&A

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