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  1. Link

    products/primary-antibodies/parathyroid-hormone-receptor-1pth1r-antibody-ab75150.pdf

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Anti-Parathyroid Hormone Receptor 1/PTH1R 抗体 (ab75150)

  • Datasheet
  • SDS
Reviews (3)Q&A (3)References (8)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)
  • Immunocytochemistry/ Immunofluorescence - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)

Key features and details

  • Rabbit polyclonal to Parathyroid Hormone Receptor 1/PTH1R
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

こちらの製品もご検討ください

一次抗体
Product image
Anti-LOX antibody [EPR4025] (ab174316)
タンパク質
Product image
Recombinant Human Parathyroid Hormone Receptor 1/PTH1R protein (His tag) (ab182670)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-Parathyroid Hormone Receptor 1/PTH1R antibody
    Parathyroid Hormone Receptor 1/PTH1R 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Parathyroid Hormone Receptor 1/PTH1R
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Thyroid axis

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Parathyroid Hormone Receptor 1/PTH1R protein (His tag) (ab182670)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75150の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/500 - 1/1000. Predicted molecular weight: 66 kDa.
IHC-P (1)
1/50 - 1/100.
ICC/IF
1/500 - 1/1000.
特記事項
WB
1/500 - 1/1000. Predicted molecular weight: 66 kDa.
IHC-P
1/50 - 1/100.
ICC/IF
1/500 - 1/1000.

ターゲット情報

  • 機能

    Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
  • 組織特異性

    Expressed in most tissues. Most abundant in kidney, bone and liver.
  • 関連疾患

    Jansen metaphyseal chondrodysplasia
    Chondrodysplasia Blomstrand type
    Enchondromatosis multiple
    Eiken skeletal dysplasia
    Primary failure of tooth eruption
  • 配列類似性

    Belongs to the G-protein coupled receptor 2 family.
  • 細胞内局在

    Cell membrane.
  • Target information above from: UniProt accession Q03431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5745 Human
    • Entrez Gene: 19228 Mouse
    • Entrez Gene: 56813 Rat
    • Omim: 168468 Human
    • SwissProt: Q03431 Human
    • SwissProt: P41593 Mouse
    • SwissProt: P25961 Rat
    • Unigene: 1019 Human
    • Unigene: 3542 Mouse
    • Unigene: 48025 Rat
    see all
  • 別名

    • MGC138426 antibody
    • MGC138452 antibody
    • Parathyroid hormone 1 receptor antibody
    • Parathyroid hormone/parathyroid hormone related peptide receptor antibody
    • Parathyroid hormone/parathyroid hormone related protein receptor antibody
    • Parathyroid hormone/parathyroid hormone-related peptide receptor antibody
    • PTH receptor antibody
    • PTH/PTHr receptor antibody
    • PTH/PTHrP receptor antibody
    • PTH/PTHrP type I receptor antibody
    • PTH1 receptor antibody
    • PTH1R antibody
    • PTH1R_HUMAN antibody
    • PTHR 1 antibody
    • PTHR antibody
    • PTHR1 antibody
    see all

画像

  • Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)
    Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)
    All lanes : Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 66 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?
    Additional bands at: 117 kDa, 82 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)This image is courtesy of an Abreview submitted by Thomas Lozito

    ab75150 staining Parathyroid Hormone Receptor 1/PTH1R in Lizard regenerated tail (Anolis sagrei) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 45 minutes at 22°C; antigen retrieval was by heat mediation in sodium citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in 1% horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

    Image is of a regenerated lizard tail cartilage tube, at a proximal region that has begun to ossify. Positive staining was observed in structures resembling embryonic bone collar and early hypertrophic chondrocytes.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)

    Immunohistochemistry analysis of Parathyroid Hormone Receptor 1/PTH1R in paraffin-embedded human brain tissue using ab75150, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

  • Immunocytochemistry/ Immunofluorescence - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)
    Immunocytochemistry/ Immunofluorescence - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150)

    Immunofluorescence analysis of Parathyroid Hormone Receptor 1/PTH1R in MCF7 cells using ab75150, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (8)

ab75150 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab75150 は 8 報の論文で使用されています。

  • Li Y  et al. Exogenous Parathyroid Hormone Alleviates Intervertebral Disc Degeneration through the Sonic Hedgehog Signalling Pathway Mediated by CREB. Oxid Med Cell Longev 2022:9955677 (2022). PubMed: 35265269
  • Chaimana R  et al. CFTR-mediated anion secretion in parathyroid hormone-treated Caco-2 cells is associated with PKA and PI3K phosphorylation but not intracellular pH changes or Na+/K+-ATPase abundance. Biochem Biophys Rep 27:101054 (2021). PubMed: 34189282
  • Chen S  et al. Preactivation of ß-catenin in osteoblasts improves the osteoanabolic effect of PTH in type 1 diabetic mice. J Cell Physiol N/A:N/A (2019). PubMed: 31301073
  • He S  et al. Parathyroid Hormone-Like Hormone Induces Epithelial-to-Mesenchymal Transition of Intestinal Epithelial Cells by Activating the Runt-Related Transcription Factor 2. Am J Pathol 188:1374-1388 (2018). PubMed: 29577935
  • Hu J  et al. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS One 9:e89352 (2014). WB, ICC/IF ; Mouse . PubMed: 24551245
  • Horie M  et al. Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen. Osteoarthritis Cartilage 20:1197-207 (2012). PubMed: 22750747
  • Cowan RW  et al. PTHrP increases RANKL expression by stromal cells from giant cell tumor of bone. J Orthop Res 30:877-84 (2012). PubMed: 22102368
  • Bond SR  et al. Pannexin 3 is a novel target for Runx2, expressed by osteoblasts and mature growth plate chondrocytes. J Bone Miner Res 26:2911-22 (2011). PubMed: 21915903

レビューと Q&A

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レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-Parathyroid Hormone Receptor 1/PTH1R antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (osteoblasts)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
40 µg
Specification
osteoblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 25 2018

Immunohistochemistry (Frozen sections) abreview for Anti-Parathyroid Hormone Receptor 1/PTH1R antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Kidney, Liver)
Permeabilization
No
Specification
Kidney, Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Daniel Tausan

Verified customer

投稿 Mar 31 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Parathyroid Hormone Receptor 1/PTH1R antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Lizard Tissue sections (Regenerated lizard tail (Anolis sagrei))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate buffer, pH 6.0
Permeabilization
No
Specification
Regenerated lizard tail (Anolis sagrei)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Thomas Lozito

Verified customer

投稿 May 06 2014

Question

What antigen retrieval method was used?

Read More

Abcam community

Verified customer

Asked on Feb 15 2012

Answer

Thank you for your phone call today and for your questions about using ab75150 in IHC.

Please keep me updated about the results using this antibody after a heat-mediated antigen retrieval with pH 6 citrate buffer. If you need any further assistance, please don't hesitate to contact me.

Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Abcam Scientific Support

Answered on Feb 15 2012

Question

Dear abcam technical support team: This customer would like to order ab12008 (Anti-RANK antibody [9A725]) and ab75150 (Anti-Parathyroid Hormone Receptor 1 antibody), She would likes to order these antibodies and test in IHC-P with rat sample, would you please help this customer to create a discount code, Could you please help this customer to solve these problems? Thanks for your kindly help.

Read More

Abcam community

Verified customer

Asked on Dec 28 2011

Answer

ab12008 : DISCOUNT CODE: *********** Expiration date: DD MM YYYY ab75150 : DISCOUNT CODE: *********** Expiration date: DD MM YYYY I am very pleased to hear your customer would like to accept our offer and test both ab12008 and ab75150 in Rat. Each code will give your customer 1 free primary before the expiration date. To redeem this offer, please submit an Abreview for Rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Dec 28 2011

Question

Testing discount for ab54186 in cow tissue.

Read More

Abcam community

Verified customer

Asked on Dec 19 2011

Answer

DISCOUNT CODE: ********Expiration date: 20April 2012 I am very pleased to hear you would like to accept our offer and test ab54186 in cow tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for cow tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

Read More

Abcam Scientific Support

Answered on Dec 19 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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