Anti-Parathyroid Hormone 抗体 [EPR9939] (ab154792)
![Western blot - Anti-Parathyroid Hormone antibody [EPR9939] (ab154792)](https://www.abcam.co.jp/ps/products/154/ab154792/Images/ab154792-161285-anti-parathyroid-hormone-antibody-epr9939-western-blot.jpg "Western blot - Anti-Parathyroid Hormone antibody [EPR9939] (ab154792)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9939] to Parathyroid Hormone
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-Parathyroid Hormone antibody [EPR9939]
Parathyroid Hormone 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9939] to Parathyroid Hormone -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human Parathyroid Hormone.
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ポジティブ･コントロール
- Tagged Parathyroid Hormone recombinant protein; Human parathyroid gland carcinoma tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9939 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154792の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
1/50000 - 1/200000. Predicted molecular weight: 13 kDa.
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| IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
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WB
1/50000 - 1/200000. Predicted molecular weight: 13 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. -
関連疾患
Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. -
配列類似性
Belongs to the parathyroid hormone family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5741 Human
- Omim: 168450 Human
- SwissProt: P01270 Human
- Unigene: 37045 Human
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別名
- hPTH antibody
- Parathormone antibody
- Parathyrin antibody
see all
画像
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Western blot - Anti-Parathyroid Hormone antibody [EPR9939] (ab154792)Anti-Parathyroid Hormone antibody [EPR9939] (ab154792) at 1/50000 dilution + Tagged Parathyroid Hormone recombinant protein at 0.01 µg
Secondary
Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 13 kDa -
![ELISA - Anti-Parathyroid Hormone antibody [EPR9939] (ab154792)](https://www.abcam.co.jp/ps/products/154/ab154792/Images/ab154792-286777-anti-parathyroid-hormone-antibody-epr9939-elisa.jpg)
ELISA - Anti-Parathyroid Hormone antibody [EPR9939] (ab154792)Direct ELISA antibody dose-response curve using recombinant anti-Human Parathyroid Hormone antibody (ab154792) against full length human parathyroid hormone protein followed Alkaline Phosphatase conjugated Goat Anti-Rabbit IgG secondary antibody.
Antigen concentration: 100 ng/ml
Primary ab concentration: 0-1000 ng/ml
Secondary ab concentration: 1:200 dilution
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [EPR9939] (ab154792)](https://www.abcam.co.jp/ps/products/154/ab154792/Images/ab154792-161284-anti-parathyroid-hormone-antibody-epr9939-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [EPR9939] (ab154792)Immunohistochemical analysis of paraffin-embedded Human parathyroid gland carcinoma tissue labeling Parathyroid Hormone with ab154792 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Parathyroid Hormone antibody [EPR9939] (ab154792)](https://www.abcam.co.jp/ps/products/154/ab154792/Images/ab154792-5-benefits-of-recombinant-antibodies.png)
Anti-Parathyroid Hormone antibody [EPR9939] (ab154792)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154792 は 1 報の論文で使用されています。
- Pan J et al. Regeneration of a Bioengineered Thyroid Using Decellularized Thyroid Matrix. Thyroid 29:142-152 (2019). PubMed: 30375266