Anti-PAR6 抗体 [EPR12378] - N-terminal (ab180159)
![Western blot - Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)](https://www.abcam.co.jp/ps/products/180/ab180159/Images/ab180159-208755-anti-par6-antibody-epr12378-n-terminal-western-blot.jpg "Western blot - Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12378] to PAR6 - N-terminal
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PAR6 antibody [EPR12378] - N-terminal
PAR6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12378] to PAR6 - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human PAR6 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NPB6 -
ポジティブ･コントロール
- HeLa, 293T, Jurkat, Y79 and fetal liver lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR12378 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180159の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
1/10 - 1/100.
|
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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| 特記事項 |
|---|
|
IP
1/10 - 1/100. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
ターゲット情報
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機能
Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. -
組織特異性
Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta. -
配列類似性
Belongs to the PAR6 family.
Contains 1 OPR domain.
Contains 1 PDZ (DHR) domain.
Contains 1 pseudo-CRIB domain. -
ドメイン
The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
The OPR domain mediates interactions with MAP2K5.
The PDZ domain mediates the interaction with CRB3. -
細胞内局在
Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions. - Information by UniProt
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参照データベース
- Entrez Gene: 50855 Human
- Entrez Gene: 56513 Mouse
- Entrez Gene: 307799 Rat
- Omim: 607484 Human
- SwissProt: Q9NPB6 Human
- SwissProt: Q9Z101 Mouse
- SwissProt: Q6B4M5 Rat
- Unigene: 112933 Human
see all -
別名
- 0710008C04Rik antibody
- 2610010A15Rik antibody
- Par 6 partitioning defective 6 C elegans homolog alpha antibody
see all
画像
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Western blot - Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)All lanes : Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal liver lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa -
![Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)](https://www.abcam.co.jp/ps/products/180/ab180159/Images/ab180159-208754-anti-par6-antibody-epr12378-n-terminal-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing PAR6, using ab180159 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)](https://www.abcam.co.jp/ps/products/180/ab180159/Images/ab180159-3-benefits-of-recombinant-antibodies.png)
Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab180159 は 3 報の論文で使用されています。
- Zhou Y et al. Congenital biliary atresia is correlated with disrupted cell junctions and polarity caused by Cdc42 insufficiency in the liver. Theranostics 11:7262-7275 (2021). PubMed: 34158849
- Johnson MB et al. Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. Nature 556:370-375 (2018). PubMed: 29643508
- Zuckerwise L et al. H19 long noncoding RNA alters trophoblast cell migration and invasion by regulating TßR3 in placentae with fetal growth restriction. Oncotarget 7:38398-38407 (2016). PubMed: 27223264