Anti-PAR6 抗体 (ab6022)

Thank you for your inquiry.

Unfortunately, we only have one antibody in our catalog at the moment that is tested to work with mouse. The immunogen of does not show a high homology with mouse but the antibody was used successfully in the following publication:

PubMed: 20643355, Figure 7 IHC.

The antibody most likely binds to the last coupleof amino acids in the immunogen. This would also mean that all three isoform should be recognized.

But this has not been experimentally tested and we do not know yetthat this antibody is good for mouse under all circumstances and applications in all mouse samples.

I am sorry I did not have positive and straight forward information for you on this occasion and hope this information is nevertheless helpful to you.

Comme convenu, la commande numéro ****** (une unité gratuite de ab6022) a été expédiée aujourd'hui. Vous devriez recevoir le produit demain. J'espère que l'anti-PAR6 ab6022 fonctionnera chez la souris. Nous serions ravis si vous pouviez nous envoyer une Abreview avec votre feedback sur l'utilisation de ab6022 chez la souris. Le lien pour soumettre cette Abreview est : https://www.abcam.com/PAR6-antibody-ab6022-reviews.html N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Thank you for contacting us. I have heard of people using anti-vimentin clone V9, to detect human tissue implanted in mice. However, this is a mouse monoclonal and our version, ab8069 is unconjugated. We do not have a version conjugated to a fluorochrome, enzyme, or other detector. An anti-mouse IgG secondary may give some staining of endogenous IgG, as you are probably aware. For detecting mouse tissue without detecting human, consider ab15630, with is also available biotinylated as ab15631 and FITC-conjugated as ab15638: https://www.abcam.com/MHC-Class-II-antibody-ER-TR3-ab15630.html The ab15630 and ab15631 have been shown to work on frozen tissue, but will not work on paraffin-embedded tissue. Ab15638 has only been tested for flow cytometry so we canot guarantee it will work for IHC. Antibody ab6022 has not been tested on mouse tissue. An alignment of the immunogenic peptide with the mouse protein shows that it is 69% identical; it is difficult to predict from this if the antibody will or will not cross-react with the mouse protein. The alignment can be found at this address for the next 24 hours: http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20070510-00025988&poll=yes I hope this helps; please do not hesitate to contact us if you have any questions.