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Anti-PAR4 抗体 (ab5787)

Submit a review Q&A (3)References (6)
Western blot - Anti-PAR4 antibody (ab5787)

    Key features and details

    • Rabbit polyclonal to PAR4
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-PAR4 antibody [EPR3992] (ab109174)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-PAR4 antibody
      PAR4 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to PAR4

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Rat

    • 免疫原

      Synthetic peptide corresponding to Human PAR4 aa 324-342.
      Sequence:

      EQLKQENKTLLKVVGQLTR


      (Peptide available as ab5853)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • WB: Rat spleen extract.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab5787の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).
    特記事項
    WB
    Use a concentration of 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).

    ターゲット情報

    • 機能

      Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.

    • 組織特異性

      Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.

    • ドメイン

      The leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.
      The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells.

    • 翻訳後修飾

      Preferentially phosphorylated at the Thr-163 by PKC in cancer cells.

    • 細胞内局在

      Cytoplasm. Nucleus. Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 2310001G03Rik antibody
        • PAR 4 antibody
        • PAR-4 antibody
        • Pawr antibody
        • PAWR_HUMAN antibody
        • PRKC Apoptosis WT1 Regulator antibody
        • PRKC apoptosis WT1 regulator protein antibody
        • Prostate apoptosis response 4 protein antibody
        • Prostate apoptosis response protein 4 antibody
        • prostate apoptosis response protein PAR-4 antibody
        • Transcriptional repressor Par-4-like protein PAWR antibody
        • Transcriptional repressor PAR4 antibody
        • WT1 Interacting Protein antibody
        see all

      画像

      • Western blot - Anti-PAR4 antibody (ab5787)
        Western blot - Anti-PAR4 antibody (ab5787)
        Anti-PAR4 antibody (ab5787) + Rat spleen extract

        Predicted band size: 41 kDa

      参考文献 (6)

      ab5787 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab5787 は 6 報の論文で使用されています。

      • Cao D  et al. Vascular Endothelial Cells Produce Coagulation Factors That Control Their Growth via Joint Protease-Activated Receptor and C5a Receptor 1 (CD88) Signaling. Am J Pathol 192:361-378 (2022). PubMed: 35144762
      • Guo H  et al. PAR-4 overcomes chemo-resistance in breast cancer cells by antagonizing cIAP1. Sci Rep 9:8755 (2019). PubMed: 31217499
      • Acharya M  et al. A complex regulatory network of transcription factors critical for ocular development and disease. Hum Mol Genet 20:1610-24 (2011). IP . PubMed: 21282189
      • Fernandez-Marcos PJ  et al. Simultaneous inactivation of Par-4 and PTEN in vivo leads to synergistic NF-kappaB activation and invasive prostate carcinoma. Proc Natl Acad Sci U S A 106:12962-7 (2009). IHC-P ; Human . PubMed: 19470463
      • Acharya M  et al. Human PRKC apoptosis WT1 regulator is a novel PITX2-interacting protein that regulates PITX2 transcriptional activity in ocular cells. J Biol Chem 284:34829-38 (2009). WB, IHC-P ; Human, Mouse . PubMed: 19801652
      • Chung H  et al. IGF-I inhibition of apoptosis is associated with decreased expression of prostate apoptosis response-4. J Endocrinol 194:77-85 (2007). PubMed: 17592023

      レビューと Q&A

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      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Question

      vielen Dank für die schnelle Antwort. Da wir auf der Suche nach einem PAR-4 Antikörper sind, der PAR-4 C-terminal von der Caspase Schnittstelle erkennt, hätte Abcam ja noch einen zur Auswahl. (PAR-4 rab polycl. ab5787; Immunogen Synthetic peptide: EQLKQENKTLLKVVGQLTR, corresponding to amino acids 324-342 of Human PAR4).
      Den hätten wir als Ersatz für den PAR-4 ab49155 bitte zugeschickt. Falls Sie noch weitere Infos benötigen stehe ich Ihnen gerne zur Verfügung.

      Read More
      Answer

      Vielen Dank für Ihre Nachricht.

      Es tut mir leid, dass Sie Probleme mit diesem PAR4-Antikörper hatten. Wie besprochen, habe ich eine kostenlose Ersatzlieferung mit ab5787für Sie in Auftrag gegeben. Sie hat die Referenznummer x und solltenach dem 1. Maibei Ihnen ankommen.

      Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

      Read More

      Question

      Would like positive and negative control tissue slides for TRAIL and PAR4. Needs blocking peptide for PAR4 antibody as it is ubiquitously expressed.

      Read More
      Answer

      Thanks for your call earlier this week and for your patience. The immunogenic peptide for the PAR4 antibody ab5787 is available as ab5853- https://www.abcam.com/PAR4-peptide-ab5853.html You can use this peptide in a blocking assay in order to demonstrate the specificity of the antibody, since there is not negative control tissue available. Please let me know if you have any questions or if there is anything else that I can do for you.

      Read More

      Question

      I have a complaint about the Par4 antibody AB5787 (Lot. 45056) which seems to bind unspecific in WB of HT22 celllysate. Our customer has reviewed your suggestions and done some additional experiments - unfortunately without success. Here are his answers to your questions: 1. This antibody has been characterized on human and rat samples for Western blot application. Other species have not been tested so we do not know if it recognizes mouse PAR4. It is expected to cross-react with mouse (100% identity with immunogen) due to sequence homology but we have no data yet. - "Now tried the antibody with proteins extracted from rat neurons. The result was as bad as before". 2. As I understand the customer has multiple bands. How much protein was loaded onto the gel? We usually recommend 20-30 ug total protein/lane. If the gel is overloaded, multiple bands can be seen. - "I loaded the gel several times with 50µg and several times with 25µg protein. At the lower loaded gel there also were mutliple bands". 3. Has the customer used protease inhibitors in the lysis buffer? No information in the e-mail. - "There was protease inhibitor cockatil in the lysis buffer". 4. It is also important to test the secondary antibody "run a secondary control" to see if the detection system works properly. Has he tested this? Or has he used the same secondary antibody with another primary antibody? What exactly the specification of the secondary antibody? - "The secondary antibody is polyclonal anti rabbit and it works great with other primary antibodies". 5. We would also recommend using positive control along with the samples. Rat tissue extracts, including testes, spleen, ovary, lung, and skeletal muscle would be good positive control. The customer should be able to see a 40 kDa and a 38 kDa protein bands. - "In cells transfected with Par4-Plasmid I cant see any bands at 40 or 38 kDa. Just the many other bands". Do you have any further suggestions?

      Read More
      Answer

      Thank you for your patience. In reviewing the information, we see Western blot with multiple bands at 40 kDa, 38 kDa and unknown at 20 kDa. It is most likely that the preparation, protocol or storage of antibody that is causing the extra bands. There may be homologous sequences in this protein because in reference papers, we noticed all the researchers have cut out the target band and did not reveal the entire blot. This occurs mostly when they want to avoid revealing multiple bands. We would suggest increasing the stringency of their washes and blocking (increase detergent/salt concentration, increase temperatures and times, while decreasing the concentration of primary antibody being used.) We would also recommend using positive controls, to show which bands are relevant. We hope this helps.

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