Anti-PAQR5 抗体 (ab79517)
Key features and details
- Rabbit polyclonal to PAQR5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAQR5 antibody
PAQR5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAQR5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide derived from the C terminal of Human PAQR5
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79517の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
ターゲット情報
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関連性
PAQR5 (Progestin and adipoQ receptor family member V) is a steroid membrane receptor which binds progesterone. It may be involved in oocyte maturation. It is an integral membrane protein and is expressed in kidney, colon, adrenal gland and lung. It belongs to the ADIPOR family. -
細胞内局在
Membrane; Multi-pass membrane protein. -
参照データベース
- Entrez Gene: 54852 Human
- Entrez Gene: 315741 Rat
- Omim: 607781 Human
- SwissProt: Q9NXK6 Human
- SwissProt: Q5PQT5 Rat
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別名
- FLJ20190 antibody
- Membrane progestin receptor gamma antibody
- mPR gamma antibody
see all
画像
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Western blot - Anti-PAQR5 antibody (ab79517)All lanes : Anti-PAQR5 antibody (ab79517) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HUVEC cells with mmunizing peptide at 10 µg
Lysates/proteins at 25 µg per lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa -
Western blot - Anti-PAQR5 antibody (ab79517)This image is courtesy of an anonymous AbreviewAll lanes : Anti-PAQR5 antibody (ab79517) at 500 µg
Lane 1 : HUVEC cell lysate (positive control)
Lanes 2-4 : Non-pregnant ovine caruncle tissue lysate
Lanes 5-7 : Day 30 of gestation ovine caruncle tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa (possible non-specific binding)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab79517 は 11 報の論文で使用されています。
- Abramicheva PA et al. Decreased renal expression of PAQR5 is associated with the absence of a nephroprotective effect of progesterone in a rat UUO model. Sci Rep 13:12871 (2023). PubMed: 37553369
- Kamińska J et al. New Insights on the Progesterone (P4) and PGRMC1/NENF Complex Interactions in Colorectal Cancer Progression. Cancers (Basel) 15:N/A (2023). PubMed: 37894441
- Sztachelska M et al. Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological Therapy. J Clin Med 11:N/A (2022). PubMed: 35956024
- Goncharov AI et al. Cytotoxic Effects of the Selective Ligands of Membrane Progesterone Receptors in Human Pancreatic Adenocarcinoma Cells BxPC3. Biochemistry (Mosc) 86:1446-1460 (2021). PubMed: 34906046
- Ponikwicka-Tyszko D et al. Mifepristone Treatment Promotes Testicular Leydig Cell Tumor Progression in Transgenic Mice. Cancers (Basel) 12:N/A (2020). PubMed: 33158280